Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Dokumenty

S0902

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

Synonim(y):

Saline-sodium citrate buffer

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(1)

About This Item

Kod UNSPSC:
41105319
NACRES:
NA.25

Postać

powder blend

pH

6.8-7.2 (20-25 °C, 263 mg/L in water)

rozpuszczalność

water: 263 mg/mL, clear, colorless

obecność zanieczyszczeń

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

temp. przechowywania

room temp

Powiązane kategorie

Opis ogólny

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Zastosowanie

Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Cechy i korzyści

  • Multi-purpose use in hybridizations
  • Easily reconstituted and diluted to any concentration needed

Komponenty

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

produkt powiązany

Numer produktu
Opis
Cennik

Kod klasy składowania

13 - Non Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Klienci oglądali również te produkty

Slide 1 of 5

1 of 5

Citrate Buffer Solution, 0.09 M

Sigma-Aldrich

C2488

Citrate Buffer Solution, 0.09 M

Hybridization Solution for Northern and Southern blotting, liquid (2x concentrate)

Sigma-Aldrich

H7140

Hybridization Solution

Formamide BioReagent, ≥99.5% (GC), for molecular biology

Sigma-Aldrich

F9037

Formamide

Denhardt’s Solution for Northern and Southern blotting, lyophilized powder

Sigma-Aldrich

D9905

Denhardt′s Solution

Phosphate-Citrate Buffer tablet

Sigma-Aldrich

P4809

Phosphate-Citrate Buffer

Protocol for the Preparation of Arabidopsis Meiotic Chromosome Spreads and Fluorescent in situ Hybridization
Bolanos V, et al.
The Plant Journal (2013)
AUTEN-67, an autophagy-enhancing drug candidate with potent antiaging and neuroprotective effects
Papp D, et al.
Autophagy, 12(2), 273-286 (2016)
M Böttger et al.
Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
Christèle Maison et al.
Nature genetics, 43(3), 220-227 (2011-02-15)
HP1 enrichment at pericentric heterochromatin is considered important for centromere function. Although HP1 binding to H3K9me3 can explain its accumulation at pericentric heterochromatin, how it is initially targeted there remains unclear. Here, in mouse cells, we reveal the presence of
Aline V Probst et al.
Developmental cell, 19(4), 625-638 (2010-10-19)
At the time of fertilization, the paternal genome lacks the typical configuration and marks characteristic of pericentric heterochromatin. It is thus essential to understand the dynamics of this region during early development, its importance during that time period and how

Protokoły

Protokoły Northern i Southern Blot

Poznaj podstawy blottingu Northern i Southern, wraz z protokołami i aplikacjami do przenoszenia makrocząsteczek na nośniki membranowe.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej