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Merck

T9285

Sigma-Aldrich

Tris-EDTA buffer solution

100X concentrate for Northern and Southern blotting

Synonim(y):

Tris-EDTA wash buffer, TE buffer solution

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About This Item

Numer MDL:
Kod UNSPSC:
41105319
Identyfikator substancji w PubChem:
NACRES:
NA.25

klasa czystości

for molecular biology

Poziom jakości

sterylność

0.2 μm filtered

Postać

liquid

pH

7.8-8.2 (1 × in water)

Zastosowanie

sample preparation

obecność zanieczyszczeń

DNase, RNase, none detected

temp. przechowywania

room temp

ciąg SMILES

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

Klucz InChI

VLEIUWBSEKKKFX-UHFFFAOYSA-N

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Opis ogólny

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

Zastosowanie

TE buffer has been used for:
  • isolate, resuspend, or store DNA and RNA for PCR or qPCR
  • gel electrophoresis, including Northern and Southern blotting
  • bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells
  • antigen retrieval for immunohistochemistry

Komponenty

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Inne uwagi

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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

nwg

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Jennifer A Black et al.
Journal of clinical microbiology, 43(7), 3110-3113 (2005-07-08)
Although plasmid-mediated AmpC beta-lactamases were first reported in the late 1980s, many infectious disease personnel remain unaware of their clinical importance. These enzymes are typically produced by isolates of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. and are
Sarah Bøje et al.
Immunology and cell biology, 94(2), 185-195 (2015-08-14)
Chlamydia is the most widespread sexually transmitted bacterial disease and a prophylactic vaccine is highly needed. Ideally, this vaccine is required to induce a combined response of Th1 cell-mediated immune (CMI) response in concert with neutralizing antibodies. Using a novel
Virtual Fluorescence Color Channels by Selective Photobleaching in Digital PCR Applied to the Quantification of KRAS Point Mutations
Schlenker F et al.
Analytical Chemistry, 93, 10538-10545 (2021)
AmpC disk test for detection of plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae lacking chromosomal AmpC beta-lactamases.
Black JA, et al.
Journal of Clinical Microbiology, 43, 3110-3113 (2005)
Kashif Asghar et al.
Experimental and therapeutic medicine, 9(3), 901-904 (2015-02-11)
Indoleamine 2,3-dioxygenase (IDO) is an immunoregulatory enzyme. It plays a key role in various malignancies, infection and autoimmune diseases. IDO induces immunosuppression through the depletion of tryptophan and its downstream metabolites. Hepatitis C virus (HCV) has infected more than 12

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