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Y1501

Yeast Synthetic Drop-out Medium Supplements

without uracil

Synonim(y):

Synthetic drop-out medium for yeast

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Gabaryty przesyłkiSKUDostępnośćCena netto
20 g
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1170,00 zł

Informacje o tej pozycji

UNSPSC Code:
12352200
NACRES:
ND.02
MDL number:

1170,00 zł


Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

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Quality Segment

form

powder

application(s)

food and beverages

Application

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Preparation Note

1.92 g will supplement 1 liter of medium.

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids are present at a concentration of 76 mg/L except for leucine, which is present at 380 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L)
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Quality Level

200

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200

Quality Level

200

Quality Level

200


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Klasa składowania

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Wykazy regulacyjne

Wykazy regulacyjne dotyczą głównie produktów chemicznych. Można w nich podawać ograniczoną liczbę informacji na temat produktów niechemicznych. Brak wpisu oznacza, że żaden ze składników nie znajduje się w wykazie. Użytkownik odpowiada za zagwarantowanie bezpiecznego i zgodnego z prawem stosowania produktu.

7783-20-2

CAS No.


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

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Protokoły

Magnetic-plasmonic nanoparticles: Synthesis and application of Ag/FeCo/Ag nanobeads for cellular organelle imaging and isolation.

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

Nanocząstki magnetyczno-plazmoniczne: Synteza i zastosowanie nanoperełek Ag/FeCo/Ag do obrazowania i izolacji organelli komórkowych.

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Produkty

Inżynieria genetyczna umożliwia ekspresję na dużą skalę i izolację rekombinowanych białek do celów badawczych.

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

Powiązane treści

Instructions


Vinicius Henrique De Oliveira et al.
Ecotoxicology and environmental safety, 202, 110917-110917 (2020-08-18)
Cadmium (Cd) is an extremely toxic environmental pollutant with high mobility in soils, which can contaminate groundwater, increasing its risk of entering the food chain. Yeast biosorption can be a low-cost and effective method for removing Cd from contaminated aqueous
Karissa L Cross et al.
mBio, 9(2) (2018-03-15)
The human oral microbiota encompasses representatives of many bacterial lineages that have not yet been cultured. Here we describe the isolation and characterization of previously uncultured Desulfobulbus oralis, the first human-associated representative of its genus. As mammalian-associated microbes rarely have
Xiyan Li et al.
PloS one, 10(12), e0146152-e0146152 (2015-12-31)
Metformin, a leading drug used to treat diabetic patients, is reported to benefit bone homeostasis under hyperglycemia in animal models. However, both the molecular targets and the biological pathways affected by metformin in bone are not well identified or characterized.



Numer pozycji handlu globalnego

SKUNUMER GTIN
Y1501-20G04061838446732
Y1501-1G04061826260470

Questions

1–3 of 3 Questions  
  1. Does the yeast synthetic drop out medium supplements without Uracil (Y1501) drop out mixture contain Adenine and at what concentration.

    1 answer
    1. Yes, this product contains Adenine at a concentration of 18 mg/L. Please see the link below to review the product datasheet. The formulation information is listed on page 2.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/132/775/y1501dat.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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