PRON-RO

Pronase

from Streptomyces griseus

• Synonim(y): non-specific protease
• Numer EC enzymu: 3.4.24.4 (BRENDA, IUBMB)
• Kod UNSPSC: 12352204

Właściwości

• pochodzenie biologiczne: Streptomyces griseus
• Poziom jakości: 100
• sterylność: non-sterile
• Formularz: lyophilized powder
• opakowanie: pkg of 1 g (10165921001); pkg of 5 g (11459643001)
• producent / nazwa handlowa: Roche
• stężenie: 0.5-2 mg/mL
• Parametry: 35-40 °C optimum reaction temp.
• metody: DNA extraction: suitable
• kolor: white
• optymalne pH: 7.0-8.0
• zakres pH: 6.0-9.0
• rozpuszczalność: water: soluble 10-20 g/L
• przydatność: suitable for molecular biology
• numer dostępu NCBI: WP_012381357.1
• numer dostępu UniProt: B1W0S5; P00776
• Zastosowanie: life science and biopharma
• obecność zanieczyszczeń: Deoxyribonuclease <0.002 U/mg ( based on lyoph.); Ribonuclease <0.002 U/mg ( based on lyoph.)
• temp. przechowywania: 2-8°C
• informacje o genach: Streptomyces griseus ... SGR_RS27460(6214424)

Opis

Opis ogólny

w temperaturze 40°C z kazeiną jako substratem, pH 7,5, co odpowiada około 1270 PU/mg lub około 25 PUK/mg.

Specyficzność

Pronase has a broad specificity, breaking down virtually all proteins into their individual amino acids; resolves carboxylic acids and alcohols.

Zastosowanie

Use Pronase to completely hydrolyze proteins in research applications. Pronase is used for the degradation of proteins during the isolation of DNA and RNA, such as in the extraction of phage DNA or the isolation of plasmid DNA. It is not necessary to let pronase self-digest prior to use. It is also used in histochemistry and cell culture for tissue dissociation in conjunction with collagenase and trypsin,[1][2][3] and for the production of glycopeptides from purified glycoproteins. It has been used for the dechorionation of Zebrafish embryos.[4]

Działania biochem./fizjol.

Pronase has wide-ranging specificity. Therefore, it is commonly used when extensive or complete degradation of proteins is required. Its applications include protein analysis in cell organelles, removal of proteins during DNA and RNA isolation, and the production of protein hydrolysates for amino acid analysis.[5] In addition, Pronase surpasses trypsin in its ability to disperse fibroblastic cell lines, excelling in both monolayer detachment and the creation of single-cell suspensions.[6]

Definicja jednostki

A unit of non-specific protease activity increases the rate of release of folin-positive amino acids and peptides from casein, at +40 °C and pH 7.5. For one unit (U) 1 μmol/min tyrosine is released, and for the unit "PU", the value is 1 μg/minute (1 U = 181 PU); for the unit "PUK", it is 0.1/minute (measured is the change in absorbance of molybdenum blue, formed by reaction with the folin ′s reagent under conditions such that 1 PUK = 50 PU).

Uwaga dotycząca przygotowania

Stabilizers: Protective effect of calcium ions

The preparation contains 20% calcium acetate for stability. It is free of starch as per the current Quality Control Procedures.The activity of a diluted solution containing 0.01–0.1 M calcium was stable over 24 hours at neutral pH at 4 to 8 °C. Pronase is also protected from heat inactivation by low levels of calcium.
Working concentration: 0.5 to 2 mg/ml
Working solution: Solvent is recommended in distilled water.
Stock solution is prepared by adding pronase powder to distilled water (10 to 20 mg/ml).
Storage conditions (working solution): -15 to -25 °C

Rekonstytucja

The lyophilizate is soluble in water (10 to 20 mg/ml).

Przechowywanie i stabilność

Store dry

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07,GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H315 - H319 - H334 - H335
• Zwroty wskazujące środki ostrożności: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Klasyfikacja zagrożeń: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash