CK-RO
Roche
Creatine Kinase (CK)
from rabbit muscle
Synonim(y):
creatine phosphokinase
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About This Item
Polecane produkty
pochodzenie biologiczne
rabbit muscle
Poziom jakości
Postać
lyophilized
opakowanie
pkg of 100 mg (10127566001)
pkg of 500 mg (10736988001)
producent / nazwa handlowa
Roche
kolor
white to light yellow
optymalne pH
6.0-7.0(reverse reaction)
9.0(forward reaction)
rozpuszczalność
water: soluble
przydatność
suitable for immunoprecipitation
numer dostępu NCBI
numer dostępu UniProt
Zastosowanie
life science and biopharma
sample preparation
obecność zanieczyszczeń
ATPase <0.001%
HK <0.001%
myokinase <0.001%
temp. przechowywania
2-8°C
informacje o genach
rabbit ... CKM(100009056)
Powiązane kategorie
Opis ogólny
at 25 °C (800 U/mg lyophilizate at 37 °C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as the activator.
Zastosowanie
Creatine kinase has been used as a component of translation buffer used in in vitro translation assay and as a component of energy regeneration mix.
Działania biochem./fizjol.
Creatine kinase (CK) is a guanidino-kinase that catalyzes the reversible phosphorylation of creatine to phosphocreatine at the expense of ATP. It plays crucial roles in rapid regeneration of ATP in cells with high demand for energy. Increased level of CK in human blood indicates diseases of the nervous system and the heart muscle, malignant hypothermia, and certain tumors. Metals such as Mg, Mn and Ca act as cofactors for CK. It also forms a substrate for protein kinase C, an important signaling molecule involved in cell growth. A product of enzymatic reaction of CK, phosphocreatine, is found to prevent HIV (human immunodeficiency virus) replication in macrophages.
Jakość
Contaminants: <0.001% ATPase, HK, and myokinase, each
Charakterystyka techniczna
Specific activity: Approximately 350U/mg lyophilizate at +25°C (800U/mg lyophilizate at +37°C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as activator.
Definicja jednostki
One unit CK activity (+25 °C) = 2.3 U (+37 °C), creatin-P and ADP as substrates, NAcCys as reactivator.
Postać fizyczna
Lyophilizate, stabilized
Uwaga dotycząca przygotowania
Storage conditions (working solution): Working solutions and storage conditions in routine applications:
Dissolve in 0.1 M imidazole buffer; aliquot and store at -15 to -25 °C.
Stable for approximately four weeks at -15 to -25 °C.
Avoid repeated freezing and thawing.
Dissolve in 0.5% NaHCO3 solution.
Do not freeze. Store protected from light.
Stable for 2 - 3 days, when stored at 2 to 8 °C.
Or
Dissolve in 30 mM glycine buffer, pH 9.0, with 0.2 mM DTT.
Stable for 2 - 3 days, when stored at 2 to 8 °C.
Dissolve in 0.1 M imidazole buffer; aliquot and store at -15 to -25 °C.
Stable for approximately four weeks at -15 to -25 °C.
Avoid repeated freezing and thawing.
Dissolve in 0.5% NaHCO3 solution.
Do not freeze. Store protected from light.
Stable for 2 - 3 days, when stored at 2 to 8 °C.
Or
Dissolve in 30 mM glycine buffer, pH 9.0, with 0.2 mM DTT.
Stable for 2 - 3 days, when stored at 2 to 8 °C.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
This page may contain text that has been machine translated.
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
Certyfikaty analizy (CoA)
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Irina M Kuznetsova et al.
Biochimica et biophysica acta, 1596(1), 138-155 (2002-05-02)
GdmCl-induced unfolding of rabbit muscle creatine kinase, CK, has been studied by a variety of physico-chemical methods including near and far UV CD, SEC, intrinsic fluorescence (intensity, anisotropy and lifetime) as well as intensity and lifetime of bound ANS fluorescence.
Aurélie M Rakotondrafara et al.
Nature protocols, 6(5), 563-571 (2011-04-30)
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FEBS letters, 439(1-2), 133-137 (1998-12-16)
The crystal structure of rabbit muscle creatine kinase, solved at 2.35 A resolution by X-ray diffraction methods, clearly identified the active site with bound sulfates surrounded by a constellation of arginine residues. The putative binding site of creatine, which is
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