C7886

Creatine Phosphokinase from bovine heart

Type III, salt-free, lyophilized powder, ≥30 units/mg protein

• Synonim(y): ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase
• Numer CAS: 9001-15-4
• Numer EC enzymu: 2.7.3.2 (BRENDA, IUBMB)
• Numer WE: 232-585-5
• Numer MDL: MFCD00130876
• Kod UNSPSC: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: bovine heart
• opis: Contains primarily isozyme MM
• typ: Type III
• Formularz: salt-free, lyophilized powder
• aktywność właściwa: ≥30 units/mg protein
• masa cząsteczkowa: 80 kDa
• temp. przechowywania: −20°C

Opis

Opis ogólny

Obszar badań: Sygnalizacja komórkowa
Fosfokinaza kreatynowa (CPK) jest enzymem wewnątrzkomórkowym występującym głównie w mięśniach szkieletowych, mózgu i mięśniu sercowym.[1] CPK składa się z dwóch podjednostek polipeptydowych, a mianowicie M (typ mięśniowy) i B (typ mózgowy). Lżejsza podjednostka jest obecna w większych ilościach.[2] Geny kodujące te podjednostki znajdują się na różnych chromosomach, z B na 14q32 i M na 19q13. Podjednostki te ułatwiają rozwój trzech specyficznych tkankowo izoenzymów: CPK-MB (mięsień sercowy), CPK-MM (mięśnie szkieletowe) i CPK-BB (mózg).[3]

Zastosowanie

Creatine Phosphokinase from bovine heart has been used:

• in the reaction mix for the import of in vitro synthesized wild-type FLAG/MYC-tagged LYR Motif Containing 7 (LYRM7-F/M) or the LFK tripeptide replaced with alanine (LFK-AAA) mutant into isolated mitochondria[4]
• in autoubiquitination assay[5]
• in the preparation of premix buffer for adenylyl cyclase activity[6]
• in the preparation of E-mix for in vitro nuclear assembly and isolation[7]
• in in vitro vesicular fusion assay.[8]
• in ATP regeneration system, to facilitate the reactivation of nonmotile axonemes.[9]
• in subcellular in vitro fusion assay of autophagosome with lysosome.[10]
• in in vitro protein translation assay.[11]
• for tATPase assay of myofibrillar protein isolated from rabbit. This assay evaluated the kinetic influence of bound creatine kinase (CK) on Ca²⁺-activated myosin ATPase.[12]
• for the enzymatic hydrolysis of protein samples during tryptophan estimation by pyrolysis gas chromatography.[13]

Creatine phosphokinase from bovine heart has been used to investigate whether endothelial cell growth is stimulated by ischemic hearts. Creatine phosphokinase from bovine heart has also been used to evaluate the effect of high but nontoxic dietary intake of copper and selenium on metabolism in calves.

Działania biochem./fizjol.

Creatine kinase plays a key role in the energy metabolism of cells with intermittently high and fluctuating energy requirements. Examples of such cells include cardiac or skeletal muscle cells and neural tissues of brain and retina. The enzyme catalyzes the reversible transfer of the phosphoryl group from phosphorylcreatine to ADP, in order to generate ATP.[14] The molecular mass of the protein is found to be approximately 80,000 Da. It is made up of 2 subunits, each having a molecular weight of 40,000 ± 2000. The lighter subunit is present in larger amounts.[2]CPK is released from the cellular cytosol into the systemic circulation due to disruption of cell membranes caused by hypoxia or other injuries.[1] Furthermore, it serves as a diagnostic indicator for rhabdomyolysis and acute myocardial infarction (AMI), along with other medical disorders such as acute muscle injury, arrhythmias, and congestive cardiac failure.[3]

Przestroga

The use of 0.1% albumin in the reaction buffer is recommended to avoid inactivation due to dilution.

Definicja jednostki

One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C.

Komentarz do analizy

Protein determined by biuret.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)