This product has not been tested for any specific application, so a specific protocol is not available. However, this material has been used successfully by a number of researchers.
Please see the link below to review publications that may be helpful:
https://www.sigmaaldrich.com/search/287810?focus=papers&page=1&perpage=30&sort=relevance&term=287810&type=citation_search
287810
2′,7′-Dichlorofluorescin Diacetate
Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress.
Synonim(y):
2′,7′-Dichlorofluorescin Diacetate, H2DCFDA, 2ʹ,7ʹ-Dichlorodihydrofluorescein Diacetate
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 100 mg | Sprawdź dostępność w koszyku | 549,00 zł |
Informacje o tej pozycji
Wzór empiryczny (zapis Hilla):
C24H16Cl2O7
Numer CAS:
Masa cząsteczkowa:
487.29
UNSPSC Code:
12352202
NACRES:
NA.25
MDL number:
Assay:
≥94% (HPLC)
Form:
crystalline solid
Storage condition:
OK to freeze, protect from light
549,00 zł
Sprawdź dostępność w koszyku
Pomoc techniczna
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Pozwól nam pomócQuality Segment
assay
≥94% (HPLC)
form
crystalline solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
solubility
DMSO: 5 mg/mL
shipped in
ambient
storage temp.
−20°C
SMILES string
Clc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)Cl)C2c4c(cccc4)C(=O)O
InChI
1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChI key
PXEZTIWVRVSYOK-UHFFFAOYSA-N
General description
Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and the nitric oxide (NO) in neutrophils and macrophages and for the determination of the overall oxidative stress in toxicological phenomena.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Detection of reactive oxygen species (ROS) and nitric oxide (•NO)
Detection of reactive oxygen species (ROS) and nitric oxide (•NO)
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Crow, J.P. 1997. Nitric Oxide 1, 145.
Ripple, M.O., et al. 1997. J. Natl. Cancer Inst. 89, 40.
Caro, A., and Puntarulo, S. 1996. Biochim. Biophys. Acta1291, 245.
Perez-Garcia, F., et al. 1996. Life Sci. 59, 2033.
Ramakrishnan, N., et al. 1996. Biochem. Pharmacol. 51, 1443.
Ferrer, A.S., et al. 1990. Anal. Biochem. 187, 129.
Ripple, M.O., et al. 1997. J. Natl. Cancer Inst. 89, 40.
Caro, A., and Puntarulo, S. 1996. Biochim. Biophys. Acta1291, 245.
Perez-Garcia, F., et al. 1996. Life Sci. 59, 2033.
Ramakrishnan, N., et al. 1996. Biochem. Pharmacol. 51, 1443.
Ferrer, A.S., et al. 1990. Anal. Biochem. 187, 129.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Ta pozycja | |||
|---|---|---|---|
| assay ≥94% (HPLC) | assay ≥97% | assay ≥95% (HPLC) | assay ≥85% (HPCE) |
| form crystalline solid | form powder | form powder or crystals | form - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| manufacturer/tradename Calbiochem® | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| solubility DMSO: 5 mg/mL | solubility - | solubility DMSO: soluble | solubility DMF: soluble, DMSO: soluble, methanol: soluble |
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
Certyfikaty analizy (CoA)
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Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| 287810-100MG | 07790788054298 |
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