MAK159
Mitochondrial Membrane Potential Kit
sufficient for 500 fluorometric tests (microplate readers)
Synonim(y):
JC-10 Assay, JC-10 Mitochondrial Membrane Potential Assay
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About This Item
Kod UNSPSC:
12161503
NACRES:
NA.25
Polecane produkty
zastosowanie
sufficient for 500 fluorometric tests (microplate readers)
metoda wykrywania
fluorometric
powiązane choroby
cancer
temp. przechowywania
−20°C
Opis ogólny
Mitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis, collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
Zastosowanie
Mitochondrial Membrane Potential Kit has been used to measure mitochondrial membrane potential.
Przydatność
This kit is suitable for the detection of Mitochondrial Membrane Potential in mammalian cells and for screening apoptosis inhibitors and activators using microplate readers.
Zasada
This kit utilizes JC-10, a superior alternative to JC-1, for determining the loss of the MMP in cells. Although JC-1 is widely used in many labs, its poor water solubility often results in precipitation in aqueous buffers when used at higher concentrations. At higher concentrations, JC-10 exhibits greater aqueous solubility than JC-1. Similar to JC-1, JC-10 is a cationic, lipophilic dye that is concentrated and forms reversible red-fluorescent JC-10 aggregates (λex = 540/λem = 590 nm) in the mitochondria of cells with a polarized mitochondrial membrane. In apoptotic cells, MMP collapse results in the failure to retain JC-10 in the mitochondria and a return of the dye to its monomeric, green fluorescent form (λex = 490/λem = 525 nm). This kit can be used for monitoring apoptosis and for screening apoptosis inhibitors and activators.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Produkty
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
Testy apoptozy komórkowej do wykrywania zaprogramowanej śmierci komórki przy użyciu aneksyny V, kaspazy i testów fragmentacji DNA TUNEL.
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