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QPBCA

Sigma-Aldrich

QuantiPro BCA Assay Kit

for 0.5-30 μg/ml protein

Synonim(y):

BCA Assay, Protein determination by BCA, bicinchoninic acid reagent

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1 KIT
2210,00 zł

2210,00 zł


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2210,00 zł

About This Item

Kod UNSPSC:
12161503
NACRES:
NA.84

2210,00 zł


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Poziom jakości

Warunki transportu

wet ice

temp. przechowywania

2-8°C

Zastosowanie

In addition to protein determination and quantification in solution,[1][2][3] the QuantiPro BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups such as sulfhydryl, N-hydroxysuccinimido carboxylate, aldehyde and hydrazide on a variety of solid supports.
The kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5 to 30 μg/mL in tube assays and 1 to 20 μg/mL in 96- or 384-well plate assays.

Cechy i korzyści

  • Accurate across a broad range of protein concentrations
  • High sensitivity; linear response form 0.5 to 30 μg/ml of protein
  • Stable color complex
  • Reduced susceptibility to detergents

Zasada

Based on the alkaline reduction of Cu2+ to Cu1+ by proteins, and the formation of a bicinchoninic acid:Cu1+ complex having an absorbance maximum at 562 nm.

Przechowywanie i stabilność

Store Reagents QA, QB, and QC at room temperature. Reagents QA and QB can be mixed together without Reagent QC (4% Copper(II) Sulfate Pentahydrate Solution 12 mL) and stored in a closed container at room temperature for a week. If this kit is received or stored cold, a precipitate may form in Reagent QA or Reagent QB. To dissolve the precipitate, warm the solution slowly to room temperature while mixing. The solutions are now suitable for use.

Informacje prawne

QuantiPro is a trademark of Sigma-Aldrich Co. LLC
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Tylko elementy zestawu

Numer produktu
Opis

  • Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL

Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki

  • P0914Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1Karta charakterystyki

Zastosowanie

Numer produktu
Opis
Cennik

produkt powiązany

Numer produktu
Opis
Cennik

Piktogramy

CorrosionEnvironment

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2

Kod klasy składowania

8A - Combustible corrosive hazardous materials


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Produkty

High-throughput proteomics advances with improved analysis methods and mass spectrometry.

Cell lysis challenges and protein extraction variables for Western blot sensitivity and reproducibility.

The possible causes and potential remedies for challenges encountered in the immunoprecipitation-Western blot technique, which consists of cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.

Możliwe przyczyny i potencjalne środki zaradcze dla wyzwań napotykanych w technice immunoprecypitacji-Western blot, która składa się z lizy komórek, tworzenia kompleksu przeciwciało-antygen (immunologicznego), wytrącania kompleksów immunologicznych i analizy metodą Western blot.

Protokoły

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Powiązane treści

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

Dostępne są produkty do tradycyjnych i alternatywnych technik kwantyfikacji białek, w tym BCA, Bradford, Lowry i innych.

Metody kwantyfikacji białek, odczynniki i technologia testów immunologicznych do dokładnego pomiaru stężenia białek w różnych próbkach.

Questions

1–2 of 2 Questions  
  1. How many samples (1 mL) can be analysed with the kit?

    1 answer
    1. Below is the link to the product bulletin. The reagents and their volumes included in the kit can be found on page 1. Table 1 on page 2 shows the volume of QuantiPro Working Reagent to prepare and the amount of each component needed:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/358/950/qpbcabul.pdf

      For 15 2 ml Standard Test tube assays, 7.75 ml of QA, 7.75 ml of QB, and 0.31 of Copper(II) Sulfate Reagent QC are needed. By calculation, about 480 assays can be done. Fewer may be gotten due to loss during pipetting. Also one may use more or less Protein Standard Solution depending upon how many assays they do each time. Protein Standard Solution (P0914) is available separately in case one needs more for their assays.

      Helpful?

  2. How many samples can be analyzed for one kit ? How long can the reagents be kept and at which temperature ?

    1 answer
    1. This kit can be used in several different formats. The format chosen, the number of blanks, and standards will determine the number of tests. The protein standard requires refrigerated storage. Other components are stable up to 1 week at room temperature. For full storage details and test count information, please refer to the technical bulletin, page 2. Click on the link below to review this document:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/358/950/qpbcabul.pdf

      Helpful?

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