MCF7 Cell Line human

86012803, human breast (adenocarcinoma), Epithelial-like

• Synonim(y): IBMF-7 Cells, MCF7 Cells, Michigan Cancer Foundation-7 Cells, ssMCF-7 Cells
• Kod UNSPSC: 41106514
• eCl@ss: 32190102

Właściwości

• product name: MCF7 Cell Line human, from human breast(adenocarcinoma), 86012803
• pochodzenie biologiczne: human breast (adenocarcinoma)
• tryb wzrostu: Adherent
• kariotyp: 2n = 46, hypertriploid to hypotetraploid
• morfologia: Epithelial-like
• produkty: Not specified
• receptory: Reported to express the oestrogen and progesterone receptor
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian breast adenocarcinoma

Opis linii komórkowej

Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.

Zastosowanie

Tumourigenicity and virus studies: Type B and C viruses

Profil DNA

STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15

pożywka hodowlana

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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