Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Dokumenty

B6916

Supelco

Bradford Reagent

for 0.1-1.4 mg/ml protein

Synonim(y):

Coomassie dye binding protein assay, Coomassie dye binding protein assay, Protein dye reagent, Protein dye reagent

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(1)

About This Item

Kod UNSPSC:
12161500
NACRES:
NA.32

Poziom jakości

200

Postać

solution

temp. przechowywania

2-8°C

Powiązane kategorie

Opis ogólny

Bradford assay is addition of coomassie brilliant blue G-250 to protein solution. The coomassie blue dye associates with basic and aromatic amino acids, thereby causing shift in absorbance during protein determination.

Zastosowanie

Bradford Reagent has been used to determine total protein concentration.

Cechy i korzyści

  • The reagent is ready to use. No mixing or dilution required.
  • Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
  • Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
  • Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.
  • Can be used in microwell plate assays.
  • Inexpensive assay.

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informacje prawne

Zastosowanie

Numer produktu
Opis
Cennik

najczęściej kupowane z tym produktem

Numer produktu
Opis
Cennik

produkt powiązany

Numer produktu
Opis
Cennik

TP0100

Total Protein Kit, Micro

Piktogramy

Health hazardCorrosion
GHS08,GHS05

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H290,H315,H319,H371

Klasyfikacja zagrożeń

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

Organy docelowe

Eyes,Central nervous system

Kod klasy składowania

8B - Non-combustible corrosive hazardous materials

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Sin-Jin Li et al.
Clinical nutrition (Edinburgh, Scotland), 36(3), 760-767 (2016-06-28)
The cellular mechanisms of obesity-induced cardiomyopathy are multiple and not completely elucidated. The objective of this study was to differentiate two obesity-associated cardiomyopathy miniature pig models: one with the metabolic syndrome (MetS), and one with a metabolically healthy obesity (MHO).
Influence of condensing equipment and temperature on exhaled breath condensate pH, total protein and leukotriene concentrations.
Czebe K et al.
Respiratory Medicine, 102, 720-720 (2008)
M Tal et al.
The Journal of biological chemistry, 260(18), 9976-9980 (1985-08-25)
Dimethyl sulfoxide was found to be effective for extraction of Coomassie Brilliant Blue R-250 (Coomassie R) from stained proteins on polyacrylamide gel slices. A good correlation was found between the ability of different proteins to bind Coomassie R and their
Role of rpoS in the development of cell envelope resilience and pressure resistance in stationary-phase Escherichia coli.
Charoenwong D et al.
Applied and Environmental Microbiology, 77, 5220-5229 (2011)
Hugh S Winwood-Smith et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 313(4), R347-R356 (2017-07-14)
Long-term studies have found that low-carbohydrate diets are more effective for weight loss than calorie-restricted diets in the short term but equally or only marginally more effective in the long term. Low-carbohydrate diets have been linked to reduced glycogen stores
Wyświetl wszystkie powiązane dokumenty

Produkty

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

Protokoły

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Przygotowanie próbki do Western Blotting

Zasady i dobre praktyki w zakresie przygotowywania próbek do Western blot z hodowli komórkowych i próbek tkanek.

Western Blotting Sample Preparation

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

Powiązane treści

Ilościowe oznaczanie białek

Metody kwantyfikacji białek, odczynniki i technologia testów immunologicznych do dokładnego pomiaru stężenia białek w różnych próbkach.

Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

Narzędzia do ilościowego oznaczania białek

Dostępne są produkty do tradycyjnych i alternatywnych technik kwantyfikacji białek, w tym BCA, Bradford, Lowry i innych.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej