Each tablet of this product should weigh between 1814.5 and 2005.5 milligrams.
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製品名
リン酸緩衝生理食塩水, tablet
フォーム
tablet
品質水準
保管条件
dry at room temperature
色
white
pH
7.2-7.6 (1 tablet/200 mL)
溶解性
water: soluble
密度
1.02 g/cm3 at 20—25 °C
1.03 g/cm3 at 20—25 °C
アプリケーション
diagnostic assay manufacturing
histology
life science and biopharma
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関連するカテゴリー
詳細
細胞生物学以外では、最適な抗体-抗原相互作用を得るためのカスタムELISAバッファーの作成などの用途で、PBSは高い汎用性を示しています。また、プロテインA/Gクロマトグラフィー用の優れたランニングバッファーとして機能し、特定の抗原結合に基づく抗体精製を促進します。血液学的および生化学的手順においては、細胞や組織の効果的な洗浄においてPBSは重要な役割を果たします。このように、PBSは、優れた等張性、緩衝能力、容易性、および安定性をもたらす、生物学的および生化学的研究に欠かせないツールとして注目されています。細胞生物学、試薬調製、タンパク質精製、および各種生物学的アッセイにおけるその多用途性は、科学上の知識と理解を深める上でPBSが極めて重要であることを浮き彫りにしています。
アプリケーション
特徴および利点
- 多数の研究用途に適した高品質な製品
- 生物学的および生化学的用途におけるバッファー構成要素として使用可能
- 高い水溶性
再構成
よく一緒に購入される製品
推奨
保管分類コード
13 - Non Combustible Solids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
P4417-BULK:
P4417-100TAB:
P4417-VAR:
P4417-50TAB:
最新バージョンのいずれかを選択してください:
試験成績書(COA)
この製品を見ている人はこちらもチェック
資料
Ensure optimal cell culture outcomes with ultrapure, endotoxin-free water. Learn about the impact of water impurities on mammalian cell cultures and how to achieve optimal cell growth.
プロトコル
Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.
Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
関連コンテンツ
表面未修飾の金ナノロッドへのカルボキシ基修飾方法、およびその金ナノロッドを用いたストレプトアビジンおよびIgGの結合について解説します。
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How much should one tablet weigh for it to yield 200ml 1xPBS? Asking just to make sure we won't use chipped tablets.
1 回答-
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How can I determine the shelf life / expiration / retest date of this product?
1 回答-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf役に立ちましたか?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 回答-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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If I want to make the same buffer molarity as the product P4474-1L, how many tablets should I dissolve in 1L of DI water?
1 回答-
P4417 and P4474 have different components and initial concentrations. One P4417 tablet dissolved in 200 mL of deionized water yields a 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride at pH 7.4, 25 °C. P4474 contains 1.058 mM potassium phosphate monobasic, 154 mM sodium chloride, and 2.97 mM sodium phosphate dibasic dihydrate. Due to these differences, it is not feasible to directly match the molarity of P4474 by dissolving P4417 tablets.
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Going by instructions, 1 tab in 200 mL water gives a 1x PBS sol'n. The recipe I use for 1x PBS has the same salt concs. Sorry if this is a silly question, but can I make up a 3x PBS solution simply by dissolving 3 tablets in 200 mL of water?
1 回答-
These tablets have not been tested for solubility or dissolution time at a 3X concentration. The end user would have to determine solubility experimentally. Note that 10X prepared solutions are available and may easily be diluted to the desired concentration. Please see the link below to review several of these product options:
https://www.sigmaaldrich.com/search/pbs-10x?focus=products&page=1&perpage=30&sort=relevance&term=PBS%2010X&type=product役に立ちましたか?
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Hi! If with this product I want to do a 10x solution, do i dilute it in 2000 mL or first in 200mL and then do the dilution? Thanks!
1 回答-
Dissolve 1 tablet in 200 ml of water to obtain: 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4 At 25 °C. These instructions are for a 1 X PBS preparation and this product is only intended for this specific concentration. If a 10X concentrate is needed, there are options in the catalog, such as the following products : 79383, P7059, 6506-OP, and 6505-OP.
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Is it possible to obtain the salt components for product 6501-200TAB? Additionally, can you provide the composition details for other PBS tablets or powder products?
1 回答-
The compositions are as follows:
1. 6501-200TAB: This PBS contains 137mM NaCl, 2.7 mM KCl, and 10mM Na phosphate.
2. P3813 - powder - Packed in Pouch or PAK: Each pouch contains 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride, totaling 9.55 g per packet. This is a standard PBS formulation.
3. P4417- Tablet: When dissolved in 200 mL of deionized water, one tablet yields 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride, at pH 7.4 and 25 °C. This product contains the same formulation as P3813 on a per liter basis (excluding tableting fillers): 8 g/L Sodium Chloride, 0.2 g/L Potassium Phosphate, monobasic, 1.15 g/L Sodium Phosphate, dibasic, and 0.2 g/L Potassium Chloride. This mixture should be diluted to one liter of water, resulting in a pH of 7.4, which can be adjusted using phosphoric acid or sodium hydroxide. The concentrations are 10 mM phosphate buffer, 2.7 mM potassium chloride, and 137 mM. These PBS tablets do not contain BSA.
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What is the osmolality of P4417 made to 1X PBS at pH 7.4?
1 回答-
This PBS once diluted in 200 mL distilled water will account for:
2.7 mM potassium chloride = 5.4 mosmoles / L
137 mM sodium chloride = 274 mosmoles / LThe exact osmolality is not determined.
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Is this PBS calcium-free?
1 回答-
The formulation of this product is considered proprietary. However, calcium is not included in the composition.
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What is the purity and CAS
1 回答-
This product is a mixture of components. CAS numbers are not assigned to multi-component products. Likewise, as there are multiple components, purity is not reported. Upon reconstitution according to instructions, the solution will contain 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride.
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