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安全性情報

P5493

Sigma-Aldrich

リン酸緩衝生理食塩水

10X, solution, pH 7.2-7.6 (25 °C), BioPerformance Certified, for molecular biology

別名:

PBS

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サイズを選択してください

1 L
¥23,800
4 L
¥80,500

¥23,800

単価¥34,000割引30%

出荷予定日2025年3月26日


バルクの問い合わせ

サイズを選択してください

表示を変更する
1 L
¥23,800
4 L
¥80,500

About This Item

MDL番号:
UNSPSCコード:
12161700
eCl@ss:
32129211
NACRES:
NA.25

¥23,800

単価¥34,000割引30%

出荷予定日2025年3月26日


バルクの問い合わせ

製品名

リン酸緩衝生理食塩水, 10× concentrate, BioPerformance Certified, suitable for cell culture

グレード

BioPerformance Certified
for molecular biology

品質水準

100
200

無菌性

0.2 μm filtered

フォーム

solution

保管条件

(Tightly closed)

テクニック

cell culture | mammalian: suitable
immunocytochemistry: suitable
immunofluorescence: suitable

不純物

Bioburden, tested
endotoxin, tested
≤5 ppm heavy metals (as Pb)

colorless

pH

7.2-7.6 (25 °C)

溶解性

water: soluble

微量陽イオン

heavy metals (as Pb): ≤5 ppm

適合性

suitable for
suitable for flow cytometry
suitable for microscopy

アプリケーション

advanced drug delivery

その他の活性

DNAse, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected

SMILES記法

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5

InChI Key

LOKCTEFSRHRXRJ-UHFFFAOYSA-I

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アプリケーション

リン酸緩衝生理食塩水は、以下の目的で使用されています:
  • ヒト歯髄幹細胞(DPSC)の細胞洗浄液として[1]
  • 解剖した脳サンプルの灌流研究に[2]
  • 免疫蛍光および免疫細胞化学的標識のためのニューロン培養細胞サンプル調製用の洗浄液として[3]
  • in vitro培養時および受精時の卵巣および精子の洗浄溶液として[4]

包装

4 Lサイズは漏出のないディスペンサー用の蛇口付ボックス入りです。

その他情報

本製品を1x濃度に希釈すると、0.01 M リン酸バッファー、0.154 M NaCl のリン酸緩衝生理食塩水が得られます。溶液のpH は7.4 になります。
メルクの一連のバイオケミカル製品に関する追加情報につきましては、こちらのフォームに記入してください。

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

P5493PROC:
P5493-1L-PW:
P5493-4L-PW:
P5493-VAR:
P5493-4L:
P5493-BULK:
P5493-PH:
P5493-1L:


最新バージョンのいずれかを選択してください:

試験成績書(COA)

Lot/Batch Number

適切なバージョンが見つかりませんか。

特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Maturation, Fertilization and Implantation of Cattle Ova Cultured in Different Media
Saif AH, et al.
The eurasia proceedings of science, technology, engineering & mathematics., 3(9), 181-188 (2018)
Differences in serum steroid hormones concentrations in spontaneously hypertensive
Kozlowska A, et al.
Physiological Research (2018)
Stephen J Bush et al.
G3 (Bethesda, Md.), 9(2), 359-373 (2018-12-12)
One of the most significant physiological challenges to neonatal and juvenile ruminants is the development and establishment of the rumen. Using a subset of RNA-Seq data from our high-resolution atlas of gene expression in sheep (Ovis aries) we have provided
Distribution and Neurochemistry of the Porcine Ileocaecal Valve Projecting Sensory Neurons in the Dorsal Root Ganglia and the Influence of Lipopolysaccharide from Different Serotypes of Salmonella spp. on the Chemical Coding of DRG Neurons in the Cell Cultures
Mikolajczyk A, et al.
International Journal of Molecular Sciences, 19(9), 2551-2551 (2018)
Diverse effect of BMP-2 homodimer on mesenchymal progenitors of different origin
Hrubi E, et al.
Human Cell : Official Journal of Human Cell Research Society, 31(2), 139-148 (2018)

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3D Spheroid BME Cell Invasion Assay utilizes specialized plates and ECM for cell aggregation and spheroid formation in vitro.

3-D Culture Matrix™ Collagen I facilitates cellular growth and differentiation in vitro, offering versatile applications in biomedical research.

関連コンテンツ

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質問

1–8/8 質問  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 回答
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      役に立ちましたか?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 回答
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      役に立ちましたか?

  3. What is the molecular weight of Product No. P5493 (Phosphate buffered saline)?

    1 回答
    1. Product P5493 is a 10× concentrate phosphate buffered saline solution, certified for bio-performance and suitable for cell culture. This solution comprises two primary ingredients diluted in water:

      - Disodium hydrogenorthophosphate with a molecular weight of 141.96 g/mol, identified by CAS number 7558-79-4.
      - Sodium chloride with a molecular weight of 58.44 g/mol, identified by CAS number 7647-14-5.

      役に立ちましたか?

  4. What is the formulation of the 10X PBS, P5493?

    1 回答
    1. The exact formulation for this 10X PBS product remains proprietary. It does not contain calcium, magnesium, or glucose. Per the product page: When diluted to a 1X concentration, this product will yield a phosphate buffered saline solution with a phosphate buffer concentration of 0.01M and a sodium chloride concentration of 0.154M. The solution pH will be 7.4.

      役に立ちましたか?

  5. What is the concentration of KCl in 10x PBS? Which phosphate salts are used and what is the concentration of each salt?

    1 回答
    1. Please see the responses to each questions below:

      Q: What is the concentration of KCl in 10x PBS?
      A: No KCl is used. P5493 contains NaCl.

      Q: Which phosphate salts are used and what is the concentration of each salt?
      A: This is specifically a sodium phosphate buffer, not a mixture of sodium phosphate and potassium phosphate buffer. Unfortunately, the detailed composition remains proprietary.

      役に立ちましたか?

  6. Does Product P5493, Phosphate buffered saline (PBS), contain calcium and magnesium?

    1 回答
    1. No, it does not contain calcium and magnesium.

      役に立ちましたか?

  7. What is the Department of Transportation shipping information for this product?

    1 回答
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      役に立ちましたか?

  8. What is the concentration of the 10X stock solution of Product P5493, Phosphate buffered saline (PBS)?

    1 回答
    1. As formulated, the 10X solution is 100 mM phosphate and 1.54 M sodium chloride.

      役に立ちましたか?

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