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由来生物
bovine liver
フォーム
buffered aqueous glycerol solution
比活性
≥1,000 units/mg protein (biuret)
保管条件
(Tightly closed)
テクニック
activity assay: suitable
その他の活性
lactic dehydrogenase, myokinase, β-NADH oxidase, nucleoside monophosphokinase and ATPase ≤0.1%
保管温度
2-8°C
関連するカテゴリー
詳細
アプリケーション
Nucleoside 5′-Diphosphate Kinase has been used:
- nucleoside diphosphate kinase (NDPK) assays[3]
- coupled NDPK-luciferase assay to determine the amounts of Ras-bound guanosine triphosphate (GTP)[4]
- non-metastatic protein 23 (NM23) growth stimulation assay[5]
- in a study to assess inhibition of type I Fc epsilon receptor mediated Ca2+ influx and mediator secretion in rat mucosal mast cells [6]
- in a study to investigate protein synthesis in rabbit reticulocytes[7]
生物化学的／生理学的作用
Nucleoside 5′-Diphosphate Kinases (NDKs) aid in the synthesis of nucleoside triphosphates (NTPs) by transferring a phosphate group from ATP to nucleoside diphosphates (NDPs).[1] NDKs provide NTPs for nucleic acid synthesis, cytidine 5′-triphosphate (CTP) for lipid synthesis, uridine 5′-triphosphate (UTP) for polysaccharide synthesis, and guanosine triphosphate (GTP) for protein elongation, signal transduction, and microtubule polymerization.[8]
単位の定義
1ユニットは、PK/LDHと共役した系において、pH 7.6、25°C、1分間で、それぞれ1.0 μmolのTDPとATPをTTPとADPに変換する酵素量です。
基質
抗体
酵素
関連製品
製品番号
詳細
価格
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
N2635-100UN:
N2635-500UN:
N2635-BULK:
N2635-VAR:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
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Shaobai Huang et al.
Annals of botany, 96(4), 703-715 (2005-07-20)
Anoxia-tolerant plant tissues synthesize a number of proteins during anoxia, in addition to the 'classical anaerobic proteins' involved in glycolysis and fermentation. The present study used a model system of rice coleoptile tips to elucidate patterns of protein synthesis in
Krisztina Takács-Vellai et al.
Cellular and molecular life sciences : CMLS, 72(8), 1447-1462 (2014-12-30)
In textbooks of biochemistry, nucleoside diphosphate conversion to a triphosphate by nucleoside diphosphate 'kinases' (NDPKs, also named NME or NM23 proteins) merits a few lines of text. Yet this essential metabolic function, mediated by a multimeric phosphotransferase protein, has effects
Sanjeev Mahanta et al.
PloS one, 3(4), e2054-e2054 (2008-05-01)
The MUC1 protein is aberrantly expressed on many solid tumor cancers. In contrast to its apical clustering on healthy epithelial cells, it is uniformly distributed over cancer cells. However, a mechanistic link between aberrant expression and cancer has remained elusive.
Rob U Onyenwoke et al.
Molecular biology of the cell, 23(2), 381-389 (2011-11-25)
AMP-activated protein kinase (AMPK) is a key energy sensor that regulates metabolism to maintain cellular energy balance. AMPK activation has also been proposed to mimic benefits of caloric restriction and exercise. Therefore, identifying downstream AMPK targets could elucidate new mechanisms
Maja Herak Bosnar et al.
Molecular and cellular biochemistry, 329(1-2), 63-71 (2009-04-18)
The family of Nm23/NDPK (nucleoside diphosphate kinase) proteins regulates a vast variety of cellular processes and, therefore, participates in important physiological events like proliferation, differentiation, molecular transport, and apoptosis. The majority of experimental data concerning this gene family has been
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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