This product may be swelled in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.9). One gram of DNA-Cellulose will swell to approximately 4 mL of wet resin.
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フォーム
lyophilized powder
標識化の程度
3-8 mg double-stranded calf thymus DNA/g
保管温度
−20°C
詳細
ウシ胸腺DNA由来デオキシリボ核酸−セルロース二本鎖は、ctDNAとしても知られており、DNA結合剤の研究用に設計されたDNAの天然供給源です。ctDNAの検出限界は約2.0ngで、光散乱強度（I(LS)）の線形範囲は0.05～1.5 mµgです。[1]
アプリケーション
ウシ胸腺DNA由来のデオキシリボ核酸−セルロース二本鎖は、さまざまなタンパク質やヌクレオチドならびに抗がん剤などの化学試薬の結合パラメーターや結合特異性を調べるために使用されます。 プルダウンアッセイやクロマトグラフィー法でよく使用されます。
品質
微量のTrisとEDTAを含有する場合があります。
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
D8515-VAR:
D8515-1G:
D8515-5G:
D8515-BULK:
D8515-10G:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
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Do you have recommendations for how to swell the DNA-cellulose and prepare it for subsequent use with DNA-binding proteins?
1 回答-
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How to dissolve this product to coat elisa plate, please?
1 回答-
This product is not tested for the suitability with coating ELISA plates. It may be helpful to read through the peer-reviewed articles associated with this product: https://www.sigmaaldrich.com/search/d8515?focus=papers&page=1&perpage=30&sort=relevance&term=d8515&type=citation_search
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How do you regenerate the column using Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose), Product D8515?
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A suggested procedure for the regeneration of a DNA-Cellulose column would be to use 5 - 10 column volumes of 2M NaCl in buffer, followed by the same volume of buffer alone.
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What buffer do you use to swell the Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose) resin, Product D8515?
1 回答-
DNA-Cellulose can be swollen in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.9). One gram of DNA-Cellulose will swell to approximately 4 mL of wet resin.
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Can Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose), Product D8515, be exposed to high salt?
1 回答-
DNA-Cellulose is stable in up to 5M NaCl. DNA-Cellulose should not be exposed to solutions of NaOH, since NaOH denatures DNA from double-stranded to single-stranded.
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product D8515, Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA, be used for purification of DNA-dependent RNA Polymerase?
1 回答-
We have not tested Product D8515, Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA for this application. However, the following references utilizes double-stranded DNA cellulose for purification of DNA-dependent RNA Polymerase:J. of Bacteriology, 172, 5624-5630 (1990)Biochem. J.,133, 201-203 (1973)
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How do you store Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose) resin, Product D8515, once it is swollen?
1 回答-
DNA-Cellulose can be stored swollen in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.5 - 8) for several months at 4°C (without a bacteriostat).
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