The purity of this product is reported as the total protein content as determined by UV absorbance. The minimum protein content is 95% (on an anhydrous basis), with the remaining 5–10% consists buffer salts such as sodium acetate and sodium chloride. Minor trace impurities may include residual proteins such as ovalbumin. Electrophoretic analysis shows lysozyme as the predominant protein, indicating relatively high purity. A quantitative purity value is not available.
L6876
リゾチーム ニワトリ卵白由来
powder or granules, ≥39,000 units/mg protein
別名:
ムコペプチドN-アセチルムラモイルヒドロラーゼ, ムラミダーゼ
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| サイズ/SKU | 在庫状況 | 単価 |
|---|---|---|
1 g | 在庫from市川市大町368 | ￥8,610 |
5 g | 在庫from市川市大町368 | ￥31,100 |
10 g | 在庫from市川市大町368 | ￥41,600 |
25 g | カートの在庫状況を確認する | ￥135,000 |
100 g | カートの在庫状況を確認する | ￥481,000 |
この商品について
CAS番号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
235-747-3
MDL number:
EC Number:
Specific activity:
≥39,000 units/mg protein
Biological source:
chicken egg white
biological source
chicken egg white
Quality Segment
form
powder or granules
specific activity
≥39,000 units/mg protein
mol wt
single-chain 14.3 kDa
technique(s)
cell based assay: suitable
input
bacteria and yeast sample(s)
suitability
suitable for cell lysis
UniProt accession no.
application(s)
cell analysis
storage temp.
room temp
Gene Information
chicken ... LYZ(396218)
Application
この酵素は、細菌の細胞壁を破壊します。スフェロプラストの調製に使用されます。
Biochem/physiol Actions
リゾチ-ムは、N-アセチルムラミン酸とN-アセチル-D-グルコサミン残基との間の、ならびにキトデキストリン内のN-アセチル-D-グルコサミン残基同士の間のβ(1→4)結合を加水分解します。 グラム陽性菌は細胞壁におけるペプチドグリカンの比率が高いため、この加水分解反応に対して高い感受性を示します。 グラム陰性菌は外膜を持ち、ペプチドグリカンの比率が低いため、この加水分解反応に対する感受性は高くありません。 しかし、これらの細胞も、菌体の外膜に存在する金属イオンをキレ-トするEDTAの存在下であれば加水分解が可能です。
リゾチ-ムは、広いpH範囲(6.0~9.0)で活性を示します。 pH 6.2(0.02 ~ 0.100 M)では、pH 9.2(0.01 ~ 0.06 M)の場合よりも、極大活性がより広いイオン強度範囲で認められます。
リゾチ-ムは、広いpH範囲(6.0~9.0)で活性を示します。 pH 6.2(0.02 ~ 0.100 M)では、pH 9.2(0.01 ~ 0.06 M)の場合よりも、極大活性がより広いイオン強度範囲で認められます。
リゾチ-ムは、N-アセチルムラミン酸とN-アセチル-D-グルコサミン残基との間の、ならびにキトデキストリン内のN-アセチル-D-グルコサミン残基同士の間のβ(1→4)結合を加水分解します。グラム陽性菌は、細胞壁におけるペプチドグリカンの比率が高いため、この加水分解反応に対して高い感受性を示します。
Features and Benefits
リゾチーム （ニワトリ卵白由来）は、独自の特性を持つ汎用性の高い酵素で、 その特性により、細胞細胞壁 破壊、ゲノムDNA抽出、質量分析の外部スタンダードなど、さまざまな用途で効果的となっています。 確実で、幅広いpH範囲で活性があり、さまざまな パッケージサイズで利用できます。
Other Notes
1 ユニットは、25 °C で pH 6.2 の緩衝液に懸濁した場合、比濁分析法（600nm）により 1 分間に 0.6 μg の Micrococcus lysodeikticus を溶解します。
より詳細な情報につきましては、www.sigma-aldrich.com/enzymeexplorerのenzymes for complex carbohydrate analysisをご覧ください。
1 of 1
当該品目 | |||
|---|---|---|---|
| description powder or granules, ≥39,000 units/mg protein | description powder (crystalline), 70000-140000 U/mg | description For use as a marker in SDS-PAGE | description dialyzed, lyophilized, powder, ~100000 U/mg |
| specific activity ≥39,000 units/mg protein | specific activity 70000-140000 U/mg | specific activity - | specific activity ~100000 U/mg |
| Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) |
| biological source chicken egg white | biological source chicken egg white | biological source chicken egg white | biological source chicken egg white |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) - | technique(s) cell based assay: suitable |
| form powder or granules | form powder (crystalline) | form powder | form powder |
| application(s) cell analysis | application(s) cell analysis | application(s) - | application(s) cell analysis |
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注意喚起語
Danger
ハザードコード
Hazard Classifications
Resp. Sens. 1
保管分類
11 - Combustible Solids
一体何が起きているの？
WGK 3
引火点 (F)
Not applicable
引火点（°C）
Not applicable
PPE（個人用保護具）
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
L6876-10G: + L6876-25G: + L6876-1G-KC: + L6876-CH: + L6876-5G: + L6876-BULK: + L6876-EW: + L6876-VAR: + L6876-100G: + L6876-1G:
jan
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Would it be possible to confirm the purity of this product? I am interested in this rather than it's enzymic activity and can't find this information. Thank you
1 回答-
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We previously stored lysozyme at -20 °C, as indicated in the previous product information sheet. Should the product now be stored at room temperature instead? How long can it be stored when resuspended and keptat -20°?
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In powder form, this product may be stored at room temperature. Prepared solutions are stable for at least 1 month at 2-8 °C. The solution stability when stored at -20 °C has not been investigaed. However, product L3790, a 10 mg/mL Lysozym solution in 25 mM sodium acetate pH 4.5 with 50% glycerol, is stable for at least 1 year at -20 °C. Please see the links below to review additional information available in the product datasheet of each product.
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We typically store our lyophilized lysozyme at -20C, but one recent order (shipped at room temperature) was left at room temperature, sealed, for several weeks. Will it still be active?
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This product is stored and shipped at ambient temperature. In the powder form, this material is quite robust and does not require controlled temperature storage. This item is stable at room temperature until the lot specific expiration date.
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I have ordered this product and stored in -70 degree C. But there is no dilution preparation method available here. Can you please tell me hot to prepare that.
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This product may be prepared in 10 mM Tris-HCl, pH 8.0 at 10 mg/mL. The material is also soluble in water at 10 mg/mL. Aqueous solutions are stable for at least one month when stored at 2-8 °C. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/938/215/l6876dat-ms.pdf役に立ちましたか？
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What's the best buffer and pH for the preparation of lysozyme stock solution, and can I incubate it at room temperature for 1 day?
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This product can be resuspended in 350 µL of STET buffer (10 mM Tris-HCl, pH 8.0, with 0.1 M NaCl,
1 mM EDTA, and 5% [w/v] TRITON® X-100). The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).During this assay, the end-user should Incubate E. coli (strain ATCC 37017) bearing the pBR322 plasmid overnight in Terrific Broth (Cat. No. T0918) with 25 µg/mL tetracycline (Cat. No. T3383) and 25 µg/mL ampicillin (Cat. No. A9518). The
optimal pH for E. coli cell lysis is 8.0 ± 0.1.16For more information please read through the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/938/215/l6876dat-ms.pdf
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What is the amino acid sequence of Product L6876, Lysozyme from chicken egg white?
1 回答-
Lysozyme is a single chain polypeptide of 129 amino acids. The amino acid sequence of chicken egg white Lysozyme is described in the Journal of Biological Chemistry, 238, 2698-2707 (1963).
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product L6876, Lysozyme from chicken egg white, be inactivated by heat?
1 回答-
Solutions of Lysozyme may be inactivated by boiling for 40 seconds at 100°C at an alkaline pH.
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Under what conditions can Product L6876, Lysozyme from chicken egg white, be used to lyse E. coli?
1 回答-
The following procedure is for the lysis of E. coli. It may be used as a guideline for other species.1. Incubate E. coli (strain ATCC 37017) bearing the pBR322 plasmid overnight in Terrific Broth (Product No. T 0918) with 25 μg/ml tetracycline (Product No T 3383) and 25 μg/ml ampicillin (Product No. A 9518).2. Centrifuge 1-2 ml samples of the overnight culture.3. Resuspend the pellets in 350 μl of STET buffer (10 mM Tris-HCl, pH 8.0, with 0.1 M NaCl, 1 mM EDTA, and 5% [w/v] TRITON® X-100).4. Add 25 μl of a freshly prepared lysozyme solution (500,000 units/ml in 10 mM Tris-HCl, pH 8.0).5. Mix by vortexing for 3 seconds.6. Incubate the lysis mixture for 30 minutes at 37 °C7. After incubation, place the tube containing the lysis mixture in a boiling water bath for exactly 40 seconds.8. Centrifuge the lysis mixture at 14,000 x g.9. Remove the pellet (cell debris) from the tube using a sterile toothpick.10. Plasmid DNA from the supernatant may then be purified and analyzed.
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How can Product L6876, Lysozyme from chicken egg white, be reconstituted and can stock solutions be prepared?
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For the lysis procedure, use a freshly prepared solution of 500,000 units/ml in 10 mM Tris-HCl, pH 8.0. The product is also soluble in water (10 mg/ml) yielding a clear to slightly hazy colorless solution. Aqueous solutions should be stable for at least one month when stored between 2-8 °C.
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