71842
KOD Hot Start Master Mix
ready-to-use solution, Ready-to-use 2X mixture, containing KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO4, optimized for convenient high fidelity PCR., suitable for PCR
別名:
High fidelity master mix, Hot start PCR, Hot start master mix
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About This Item
UNSPSCコード:
41105500
NACRES:
NA.55
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品質水準
形状
ready-to-use solution
使用法
sufficient for 100 reactions
sufficient for 500 reactions
特徴
Difficult Templates/Specialty Enzymes PCR
High Fidelity PCR
dNTPs included
hotstart
メーカー／製品名
Novagen®
保管条件
OK to freeze
テクニック
PCR: suitable (2X PCR Master Mix for high fidelity)
PCR: suitable
入力
purified DNA
輸送温度
dry ice
保管温度
−20°C
詳細
KOD Hot Start Master Mix is a ready-to-use 2X mixture optimized for convenient high-fidelity PCR, which utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. It contains KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure dNTPs, and reaction buffer with MgSO4. The mix is ideal for use in high-throughput PCR applications.
KOD Hot Start Master Mix is a ready-to-use 2X mixture optimized for convenient high-fidelity PCR, which utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. The mix contains KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultra-pure deoxynucleotides, and reaction buffer with MgSO4. The monoclonal antibodies inhibit the DNA polymerase and 3′→5′ exonuclease activities at ambient temperatures.
アプリケーション
KOD Hot Start Master Mix has been used:
- To yield PCR product for T7 endonuclease I (T7EI) assay.
- For PCR amplification of the DNA isolated from Chrysosporium multifidum, a fungus with antimicrobial activity.
- In the PCR amplification of cDNA.
- In two-step PCR for the amplification of the target sequence followed by its insertion into a vector.
特徴および利点
- Highest accuracy, yield, and processivity of commercially available proofreading DNA polymerases
- Amplifies genomic DNA templates up to 12 kbp
- Amplifies plasmid and lambda DNA template up to 21 kbp
- Eliminates mispriming and primer-dimer formation
- Convenient ambient-temperature setup compatible with automation
- KOD HotStart® Buffer ensures optimal PCR performance over a wide range of targets.
- Offers high specificity, fidelity and yield during PCR amplification
- Optimized for ease of use and data reproducibility
- Simplifies PCR set-up and saves time
- High consistency with minimal risk of contamination
*Manufactured by Toyobo and distributed by Merck KGaA, Darmstadt, Germany and/or its affiliates (not in Japan).
Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
構成
- 2 × 1.25 ml or 10 × 1.25 mL KOD Hot Start Master Mix
References:
Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem.66 (10), 2194. Fujii, S., et al. 1999. J. Mol.
警告
Toxicity: Standard Handling (A)
単位の定義
One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), and 150 µg/ml activated calf thymus DNA.
その他情報
Simply add KOD HotStart® Master Mix to an equal volume of sample containing DNA template and primers. The final diluted reaction contains 1U KOD HotStart® DNA Polymerase per 50 μl reaction. The smaller available size provides sufficient master mix for 100 (50 μl scale), or 250 (20 μl scale) reactions, while the larger size is adequate for 500 (50 μl scale) or 1250 (20 μl scale) reactions.
法的情報
* Manufactured by Toyobo and distributed by Merck KGaA, Darmstadt, Germany and/or its affiliates (not in Japan).Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
関連製品
製品番号
詳細
価格
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
71842-PP:
71842-PP-15ML:
71842-1.25EA:
71842-EA:
71842-4:
71842-3:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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資料
PCRマスターミックスは、多数のPCR反応チューブに分割できるPCR試薬またはRT-PCR試薬の混合物です。通常は、DNAポリメラーゼ、dNTP、MgCl2、バッファーが含まれます。独自のマスターミックスを調製するか、市販のものを選択できます。
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
関連コンテンツ
Efficiently amplify your samples with confidence using KOD One™ PCR Master Mix for ultra-fast PCR with one of the fastest elongation rates available for high fidelity polymerases.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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