R7647
Random Nonamers
for use as primers in cDNA synthesis, 50 μM in H2O
Sinonimo/i:
Oligodeoxynucleotides
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100 μL
167,00 €
167,00 €
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Cambia visualizzazione
100 μL
167,00 €
About This Item
Codice UNSPSC:
41105500
NACRES:
NA.31
167,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Prodotti consigliati
Grado
Molecular Biology
for molecular biology
Stato
liquid
impiego
0.1 mL sufficient for 100 RT-PCR reactions
Concentrazione
50 μM in H2O
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Descrizione generale
Random Nonamers are random sequences of nine deoxyribonucleotides (9-mers) used to prime mRNA, with or without a poly(A) tail. Random nonamers may be used as universal, non-specific primers.
Applicazioni
Random Nonamers are suitable for:
- First-strand cDNA synthesis
- Preparing cDNA libraries (with oligo (dT) primers
- Prepare labeled DNA probes for hybridizations
Caratteristiche e vantaggi
- When preparing cDNA libraries or dealing with situations where specific primers are not suitable, the random nonamers can be combined with anchored oligo (dT)23 primers (Product No. O4387).
- In cases of incomplete or missing sequence information, or when specific primers are not effective, the random nonamers offer a solution for cDNA library preparation.
- The random nonamers′ priming capability at higher temperatures (up to 65°C) is reduced, ensuring that they do not interfere with polymerase chain reaction(PCR) following transcription.
- Randomnonamers can also be used to prepare labeled DNA probes for hybridizationstudiesRandom nonamers serve as alternatives to specific reverse transcription (RT) primers for first-strand synthesis, cDNA library construction, and other applications.
Componenti
Random Nonamers are provided as a 50 uM solution in water.
Altre note
For laboratory use only. Not for drug, household, or other uses.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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