M8787

Cloruro di magnesio

PCR Reagent, 25 mM MgCI₂ solution for PCR

• Sinonimo/i: PCR optimization
• Formula condensata: MgCl₂
• Numero CAS: 7786-30-3
• Peso molecolare: 95.21
• Numero MDL: MFCD00011106
• Codice UNSPSC: 12352302
• ID PubChem: 57654422
• NACRES: NA.52
• Grado: PCR Reagent
• Stato: liquid

Proprietà

• Grado: PCR Reagent
• Livello qualitativo: 200; 300
• Stato: liquid
• Confezionamento: vial of 1.5 mL
• Concentrazione: 25 mM±1 mM
• tecniche: PCR: suitable
• Colore: colorless
• applicazioni: agriculture; diagnostic assay manufacturing
• Attività estranea: DNase, RNase, none detected
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: Cl[Mg]Cl
• InChI: 1S/2ClH.Mg/h2*1H;/q;;+2/p-2; TWRXJAOTZQYOKJ-UHFFFAOYSA-L

Descrizione

Descrizione generale

Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield.

Applicazioni

Magnesium chloride solution has been used as a component of the:

• amplification mixture for polymerase chain reaction (PCR)[1]
• amplification mixture in an inter simple sequence repeats (ISSR)-PCR[2]
• reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP)[3]
• PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA[4]
• reaction mix for RT-PCR to assess the gene expression[5]

Compatibilità

Suitable for optimization of polymerase chain reactions.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)