Hexanucleotide primers are a mixture of random 5′-hydroxyl hexanucleotides or hexamers, and can be used to quickly and efficiently prepare radioactive or non-radioactive probes using a DNA polymerase and a suitable DNA template.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(16), 6404-6412 (2003-07-25)
Multiple evidence suggests that fibroblast growth factors (FGFs), most prominently FGF-2, affect astroglial proliferation, maturation, and transition to a reactive phenotype in vitro, and after exogenous administration, in vivo. Whether this reflects a physiological role of endogenous FGF is unknown.
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 10-10 (1989)
Methods for non-radioactive labeling of nucleic acids.
Kessler, C. et al.
Nonisotopic Probing, Blotting, and Sequencing, 51-54 (1995)
A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described. DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I
The Journal of physiology, 597(1), 137-149 (2018-10-20)
At the end of pregnancy, the uterus transitions from a quiescent state to a highly contractile state. This transition requires that the uterine (myometrial) smooth muscle cells increase their excitability, although how this occurs is not fully understood. We identified
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