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L7658

Sigma-Aldrich

LB Broth (Lennox)

EZMix powder microbial growth medium

Sinonimo/i:

Lennox broth

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
41106200
NACRES:
NA.85

Grado

for molecular biology

Livello qualitativo

Descrizione

quick-dissolve, dust-free formulation

Sterilità

non-sterile

Forma fisica

powder

tecniche

microbiological culture: suitable

pH

6.8-7.2(2.5% solution)

applicazioni

agriculture
food and beverages
microbiology

Temperatura di conservazione

room temp

Compatibilità

nonselective for Escherichia coli
nonselective for coliforms

Descrizione generale

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation.

Applicazioni

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Caratteristiche e vantaggi

Lennox LB EZMix powder provides:
  • Granulated, dust-free format for safer handling and faster mixing
  • Convenient small package to eliminate weighing
  • Easy scale-up using larger package sizes
  • Standard formulation

Componenti

*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.

10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
0.6 g/L inert binder (EZMix formulation only)

Nota sulla preparazione

1. Suspend 20.6 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1M magnesium sulfate after autoclaving.

Ricostituzione

Stir to suspend 20.6g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Note legali

Zeocin is a registered trademark of Cayla Sarl

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Protocolli

General protocols for growth of competent cells in microbial medium.

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