CMC0017
OverExpress™ C41(DE3) Chemically Competent Cells
Escherichia coli, rod shaped
Sinonimo/i:
C41 strain
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About This Item
Codice UNSPSC:
41106202
NACRES:
NA.85
Prodotti consigliati
product name
OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression
Origine biologica
Escherichia coli
Grado
for molecular biology
Modalità di accrescimento
adherent or suspension
Morfologia
rod shaped
tecniche
microbiological culture: suitable
Trasformazione cellulare
competent cell type: chemically competent
transformation efficiency: ≥1 x 106 cfu/μg
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Descrizione generale
OverExpress™ Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress™ strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Applicazioni
OverExpress™ C41 (DE3) chemically competent cells have been used for expression screening.
Caratteristiche e vantaggi
- Express genes cloned into any T7 vector with these BL21(DE3) derivatives
- Effective in expressing toxic & membrane proteins
- Cited in over 350 research articles
Componenti
- OverExpress C41(DE3) chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for expression
Note legali
OverExpress is a trademark of Imaxio, S.A.
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10 - Combustible liquids
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Protocolli
Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.