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L3397

Sigma-Aldrich

LB Broth (Luria low salt)

Powder microbial growth medium

Sinonimo/i:

Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)

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About This Item

Codice UNSPSC:
41106200
NACRES:
NA.85

Grado

for molecular biology

Livello qualitativo

200

Sterilità

non-sterile

Forma fisica

powder

Composizione

NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

tecniche

microbiological culture: suitable

pH

6.8-7.2(1.55% solution)

Compatibilità

suitable for molecular biology
nonselective for Escherichia coli
nonselective for coliforms

applicazioni

food and beverages
microbiology

Temperatura di conservazione

room temp

Categorie correlate

Descrizione generale

Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.

Applicazioni

LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.

Caratteristiche e vantaggi

Luria′s low salt LB powder provides:
  • Standard formulation
  • A budget-friendly alternative to liquid
  • Easy scale-up using larger package sizes

Nota sulla preparazione

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Ricostituzione

Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Protocolli

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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