Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
250 g | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 86,70 € |
1 kg | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 297,00 € |
Informazioni su questo articolo
86,70 €
grade
Molecular Biology
Quality Segment
sterility
non-sterile
form
powder
composition
Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Application
Si usa anche per la coltura non selettiva di ceppi di E. coli per il clonaggio, la produzione di DNA plasmidico e di proteine ricombinanti. Con l′aggiunta degli antibiotici appropriati, inclusi quelli che necessitano di un basso tenore di sali come la Zeocin®, può essere utilizzato anche per la coltura selettiva.
Features and Benefits
- Una economica alternativa alle piastre preriempite
- Facile aumento graduale con l′uso di confezioni più grandi
- Formulazione standard
Preparation Note
2. Portare all′ebollizione mescolando per sciogliere completamente tutti gli ingredienti.
3. Sterilizzare in autoclave per 15 minuti a 121 °C.
Per preparare il Lennox L Agar: Aggiungere 1 g di glucosio e procedere con le istruzioni per la preparazione come sopra. Per preparare il terreno di Enquist e Sternberg: In asepsi, aggiungere 10 ml di solfato di magnesio 1 M sterile dopo autoclavaggio.
Legal Information
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Questo articolo | |||
|---|---|---|---|
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| form powder | form powder | form tablet | form powder |
| composition Agar, 15 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L , NaCl, 5 g/L | composition Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L | composition - | composition NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| application(s) food and beverages | application(s) agriculture | application(s) food and beverages | application(s) food and beverages |
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Classe di stoccaggio
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the Department of Transportation shipping information for this product?
1 risposta-
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Which bacterial culture medium is the best choice for my application?
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Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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Will adding magnesium to the culture medium increase cell density?
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In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What is the difference among the many LB - Lennox products?
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LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
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What are the differences among the Luria, Lennox and Miller LB formulations?
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LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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