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L7275

Sigma-Aldrich

LB Broth (Lennox)

Tablet microbial growth medium

Sinonimo/i:

Lennox broth , Luria Bertani Broth

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.85

Grado

for molecular biology

Sterilità

non-sterile

Forma fisica

tablet

tecniche

microbiological culture: suitable

applicazioni

food and beverages
microbiology

Temperatura di conservazione

room temp

Compatibilità

nonselective for Escherichia coli
nonselective for coliforms

Descrizione generale

Dehydrated Luria-Bertani (LB) broth is called the Lennox LB, which has amino acids as carbon source.
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

Applicazioni

LB Broth (Lennox) has been used for culturing Escherichia coli and bacterial artificial chromosomes (BACs).
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Caratteristiche e vantaggi

Lennox LB tablets provide:
  • Convenient small package to eliminate weighing
  • Fast preparation – no stirring required

Componenti

10 g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
2.2 g/L inert binding agents

Ricostituzione

Add each 1.1g tablet in 48.3mL water (no stirring required). Autoclave for 20 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Note legali

Zeocin is a registered trademark of Cayla Sarl

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Protocolli

General protocols for growth of competent cells in microbial medium.

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