LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
L7025
LB Broth with agar (Lennox)
Tablet microbial growth medium
Sinonimo/i:
LB Agar, LB agar
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Scegli un formato
100 TABS
123,00 €
500 TABS
422,00 €
123,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Scegli un formato
Cambia visualizzazione
100 TABS
123,00 €
500 TABS
422,00 €
About This Item
Codice UNSPSC:
12352200
NACRES:
NA.85
123,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Grado
Molecular Biology
for molecular biology
Sterilità
non-sterile
Stato
tablet
tecniche
microbiological culture: suitable
applicazioni
food and beverages
microbiology
Temperatura di conservazione
room temp
Compatibilità
nonselective for Escherichia coli
nonselective for coliforms
Descrizione generale
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
Applicazioni
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Caratteristiche e vantaggi
Lennox LB tablet with agar provides:
- A budget-friendly alternative to pre-poured plates
- Convenient small package to eliminate weighing
- Fast preparation – no stirring required
Componenti
13.72g/L Agar
9.14g/L Tryptone
4.57 g/L Yeast Extract
4.57 g/L NaCl
1.6g/L inert binding agents
9.14g/L Tryptone
4.57 g/L Yeast Extract
4.57 g/L NaCl
1.6g/L inert binding agents
Ricostituzione
Add each 1.68 g tablet to 48.3 mL water (no stirring required). Autoclave for 20 minutes at 121 °C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.
Note legali
Zeocin is a registered trademark of Cayla Sarl
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Certificati d'analisi (COA)
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Protocolli
General protocols for growth of competent cells in microbial medium.
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What is the difference among the many LB - Lennox products?
1 risposta-
Utile?
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Will adding magnesium to the culture medium increase cell density?
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In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
Utile?
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Which bacterial culture medium is the best choice for my application?
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Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
Utile?
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What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile?
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What are the differences among the Luria, Lennox and Miller LB formulations?
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LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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