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L2646

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-M, lyophilized powder

Sinonimo/i:

Phaseolus vulgaris agglutinin, PHA

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10 MG
57,60 €
25 MG
95,00 €
100 MG
290,00 €

57,60 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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Scegli un formato

Cambia visualizzazione
10 MG
57,60 €
25 MG
95,00 €
100 MG
290,00 €

About This Item

Numero CE:
232-718-7
Codice UNSPSC:
12352202
NACRES:
NA.32

57,60 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Livello qualitativo

300

Stato

lyophilized powder

Potenza

<40 μg per mL agglutination activity

Composizione

Protein, 50-70% biuret

Impurezze

salt, free

Temperatura di conservazione

2-8°C

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Categorie correlate

Azioni biochim/fisiol

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Risultati analitici

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
0000334550
0000334550
0000287007
0000287006
0000229856

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Articoli

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Domande

  1. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 risposta

    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

      Utile?

Recensioni

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