11082132001
Roche
Phytohemagglutinin-M (PHA-M)
from Phaseolus vulgaris
Sinonimo/i:
PHA-M
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
23201100
Prodotti consigliati
Livello qualitativo
Sterilità
non-sterile; 0.2 μm filtered
Forma fisica
lyophilized
Confezionamento
pkg of 20 mg
Produttore/marchio commerciale
Roche
applicazioni
hematology
Temperatura di conservazione
2-8°C
Categorie correlate
Descrizione generale
Phytohemagglutinin (PHA), the lectin extract from the red kidney bean. PHA consists of five isolectins (L4E0, L3E1, L2E2, L1E3, L0E4) each being a tetramer held together by noncovalent forces. The subunits L (leukocyte reactive) have a high affinity for lymphocyte surface receptors, but little for those of erythrocytes, and are responsible for the mitogenic properties of the isolectins. E (erythrocyte reactive) is responsible for the erythrocyte-agglutinating properties.
Applicazioni
Phytohemagglutinin (PHA) is a potent mitogen used to stimulate cell proliferation in lymphocyte cultures.
Phytohemagglutinin-M (PHA-M) has been used in the activation of Jurkat and primary cells. It has also been used to treat polymorphonuclear blood cells(PMBCs)
Azioni biochim/fisiol
Phytohemagglutinin (PHA) has mitogenic and potent cell agglutinating activities. It binds to T-cell membranes and stimulates cell division and metabolic activity. PHA has the ability to stimulate close contacts between cell membranes.
Specifiche
Biological activity: <10 μg/ml for maximal stimulation of DNA synthesis (BrdU incorporation) with human peripheral blood lymphocytes.
Nota sulla preparazione
Working concentration: 2 - 10 μg/ml
Approximately 2 - 10 μg/ml for the stimulation of peripheral blood lymphocytes
Storage conditions (working solution): The reconstituted solution is stable for 2 weeks at 2 to 8 °C or for several months when stored in aliquots at -15 to -25 °C.
Approximately 2 - 10 μg/ml for the stimulation of peripheral blood lymphocytes
Storage conditions (working solution): The reconstituted solution is stable for 2 weeks at 2 to 8 °C or for several months when stored in aliquots at -15 to -25 °C.
Ricostituzione
PHA-M should be reconstituted in sterile double-dist. water (final concentration: 2–10 mg/ml). Further dilution with medium or PBS (phosphate buffered saline).
Altre note
For life science research only. Not for use in diagnostic procedures.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
Certificati d'analisi (COA)
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