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L4144

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture

Sinonimo/i:

Phaseolus vulgaris agglutinin, PHA

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5 MG
563,00 €

563,00 €

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5 MG
563,00 €

About This Item

Numero CE:
232-718-7
Codice UNSPSC:
12352202
NACRES:
NA.32

563,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Livello qualitativo

200

Nome Commerciale

BioReagent

Stato

lyophilized powder

Potenza

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.)
≤1.0 μg per mL leucoagglutination activity
≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

tecniche

cell culture | mammalian: suitable

Impurezze

salt, free

Solubilità

DPBS: 5 mg/mL, slightly hazy, colorless

Compatibilità

leucocyte agglutination activity tested

Temperatura di conservazione

2-8°C

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Descrizione generale

Lectins are carbohydrate-binding proteins,[1] which contains four subunits with a molecular weight of 125 kDa.[2] Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system.[3]

Applicazioni

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • it has been used as a positive control to treat cells[4]
  • to stimulate cell culture for the analysis of lymphocyte proliferation[5]
  • to stimulate T cells[6]

Azioni biochim/fisiol

Lectin agglutinates cells and precipitates polysaccharides and glycoproteins.[1] It is associated with toxic allergens and hemagglutinins. Lectin exhibits anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting.[7]
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Risultati analitici

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
SLCL9657
SLCB6611
SLBV1874
SLBM8615V
SLBF5256V

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The Lectins: Properties, Functions, and Applications in Biology and Medicine (2012)
Transfection of FcgammaRIIIa (CD16) Alone Can Be Sufficient To Enable Human alphabetaTCR T Lymphocytes To Mediate Antibody-Dependent Cellular Cytotoxicity
Ollier J, et al.
ImmunoHorizons, 1(5), 63-70 (2017)
Novel DOCK8 gene mutations lead to absence of protein expression in patients with hyper-IgE syndrome
Qin T, et al.
Immunologic Research, 64(1), 260-271 (2016)
SARS-CoV-2 vaccination in rituximab-treated patients: B cells promote humoral immune responses in the presence of T-cell-mediated immunity.
Mrak, et al.
Annals of the Rheumatic Diseases (2021)
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Scientific reports, 11(1), 13664-13664 (2021-07-03)
Mumps is nowadays re-emerging despite vaccination. The contribution of T cell immunity to protection against mumps has not been clearly defined. Previously, we described a set of 41 peptides that were eluted from human leukocyte antigen (HLA) class I molecules
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Articoli

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Domande

  1. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 risposta

    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

      Utile?

Recensioni

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