11249738001

Phytohemagglutinin-L (PHA-L)

from Phaseolus vulgaris

• Sinonimo/i: PHA-L
• Codice UNSPSC: 23201100

Proprietà

• Livello qualitativo: 100
• Sterilità: non-sterile; 0.2 μm filtered
• Saggio: >95% (SDS-PAGE)
• Stato: lyophilized (clear, colorless solution after reconstitution)
• Confezionamento: pkg of 5 mg
• Produttore/marchio commerciale: Roche
• applicazioni: hematology
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

PHA-L is purified from Phaseolus vulgaris (red kidney bean) by standard chromatographic techniques.
Phytohemagglutinin (PHA), the lectin extract from the red kidney bean, consists of only L-type subunits (isolectin L4, "leuko-agglutinin"). It is an N-acetylgalactosamine/galactose sugar-specific lectin. PHA consists of five isolectins (L4E0, L3E1, L2E2, L1E3, L0E4) each being a tetramer held together by noncovalent forces. The subunits L (leukocyte reactive) have a high affinity for lymphocyte surface receptors, but little for those of erythrocytes, and are responsible for the mitogenic properties of the isolectins. E (erythrocyte reactive) is responsible for the erythrocyte-agglutinating properties.[1]

Applicazioni

Phytohemagglutinin-L (PHA-L) consists of only L-type subunits (isolectin L₄, "leuko-agglutinin"), and is well suited for high-efficiency induction and the functional analysis of T-lymphocytes.[2] It is highly purified and tested for high-efficiency stimulation of human lymphocytes. It is also used as positive controls for peripheral blood mononuclear cells (PBMC) stimulation.[3][4]

Azioni biochim/fisiol

Phytohemagglutinin (PHA) has mitogenic and potent cell agglutinating activities. It binds to T-cell membranes and stimulates cell division and metabolic activity. PHA has the ability to stimulate close contacts between cell membranes.[1]

Specifiche

Biological activity: <5μg/ml for maximal stimulation of DNA synthesis (BrdU incorporation) with human peripheral blood lymphocytes

Sequenza

PHA-L is a tetramer of 4 L-type subunits held together by noncovalent forces.

Nota sulla preparazione

Working concentration: 1 to 5 μg/ml
Approximately 1 to 5 μg/ml for the stimulation of human peripheral blood lymphocytes
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable