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F8666

Sigma-Aldrich

Lipopolisaccaridi

FITC conjugate

Sinonimo/i:

LPS

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5 MG
470,00 €
10 MG
570,00 €

470,00 €

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5 MG
470,00 €
10 MG
570,00 €

About This Item

Numero CAS:
93572-42-0
Numero CE:
297-473-0
Numero MDL:
MFCD00164401
Codice UNSPSC:
12352201
NACRES:
NA.25

470,00 €

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Origine biologica

Escherichia coli (O55:B5)

Livello qualitativo

200

Coniugato

FITC conjugate

Stato

lyophilized powder

Grado di funzionalizzazione

2-10 μg FITC per mg LPS

Colore

yellow to orange

Solubilità

H2O: 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Categorie correlate

Descrizione generale

This product is a TCA extract of E. coli serotype O55:B5 (strain CDC 1644-70), which was purified by gel filtration and conjugated to FITC (fluorescein isothiocyanate).

Applicazioni

I lipopolisaccaridi (LPS) sono componenti caratteristici della parete cellulare dei batteri gram negativi. Il LPS e la sua frazione lipide A stimolano le cellule del sistema immunitario innato attraverso il recettore Toll-like 4 (TLR4), un elemento della famiglia delle proteine recettoriali Toll-like, che riconosce i profili molecolari associati ai patogeni (PAMP) comuni.
FITC-Lipopolysaccharides (FITC-LPS) may be used to study the movement/transport of LPS across tissue barriers such as the colonic epithelium and cellular binding and internalization of lipopolysaccharides with fluorescence-based assay systems.[1][2] FITC-LPS may be used to evaluate, qualify and quantify lipopolysaccharides (LPS) removal reagents and procedures.[3] FITC-LPS conjugate may be used for the detection of LPA binding proteins and motifs.[4]

Azioni biochim/fisiol

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Nota sulla preparazione

The product is soluble in water (5 mg/ml), yielding a hazy faint yellow solution. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Altre note

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Pittogrammi

Skull and crossbones
GHS06

Avvertenze

Danger

Indicazioni di pericolo

H300

Classi di pericolo

Acute Tox. 2 Oral

Codice della classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000367380
0000130338
0000367380
0000119071
0000093835

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Tatyana Y Postnikova et al.
Pharmaceuticals (Basel, Switzerland), 13(3) (2020-03-22)
Infectious diseases in early postnatal ontogenesis often result in cognitive impairments, particularly learning and memory. The essential foundation of learning and memory is long-term synaptic plasticity, which depends on N-methyl-D-aspartate (NMDA) receptors. In the present study, bacterial infection was modeled
Elena Topchiy et al.
PloS one, 11(5), e0155030-e0155030 (2016-05-14)
Sepsis is the leading cause of death in critically ill patients. While decreased Proprotein Convertase Subtilisin/Kexin type 9 (PCSK9) function improves clinical outcomes in murine and human sepsis, the mechanisms involved have not been fully elucidated. We tested the hypothesis
Yang Yu et al.
Scientific reports, 6, 20845-20845 (2016-02-10)
Phospholipid transfer protein (PLTP) participates in high density lipoprotein (HDL) metabolism. Increased plasma PLTP activity was observed in lipopolysaccharide (LPS) triggered acute inflammatory diseases. This study aimed to determine the exact role of PLTP in LPS induced inflammation. HDL pool
C Ganesh Kumar et al.
Enzyme and microbial technology, 55, 113-120 (2014-01-15)
In an ongoing survey for bioactive potential of microorganisms from different biosphere zones of India, a promising Kocuria rosea strain BS-1 was identified which produced an exopolysaccharide (designated as Kocuran) exhibiting in vitro antioxidant and immunosuppression properties. Kocuran was characterized
Feifei Wang et al.
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi, 30(3), 635-640 (2013-07-20)
In order to remove the endotoxin from the blood of endotoxemia patients, we prepared a new adsorbent with heparin space arm and polymyxin B (PMB) ligand. The carrier of chloromethyl polystyrene resin was activated and heparin space arm was grafted
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Articoli

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Domande

  1. What is the molecular weight?

    1 risposta

    1. The molecular weight of this product is not determined. However, LPS is heterogeneous and tends to form aggregates of varying sizes that may range from 1-4 million or greater. When treated with SDS and heat the molecular weight range is approximately 50 to 100 kDa. The FITC used to prepare this product has a molecular weight of 389.38 g/mol. There are approximately 3.6 ug FITC per milligram LPS in this preparation.

      Please see the link below to learn more about Lipopolysaccharides:
      https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

      Utile?

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