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F8666

Lipopolisaccaridi

FITC conjugate

Sinonimo/i:

LPS

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Voi/SKUDisponibilitàPrezzo
5 mg
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
492,00 €
10 mg
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
596,00 €

Informazioni su questo articolo

Numero CAS:
93572-42-0
UNSPSC Code:
12352201
EC Number:
297-473-0
NACRES:
NA.25
MDL number:
MFCD00164401

492,00 €

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biological source

Escherichia coli (O55:B5)

Quality Segment

200

conjugate

FITC conjugate

form

lyophilized powder

extent of labeling

2-10 μg FITC per mg LPS

color

yellow to orange

solubility

H2O: 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow

shipped in

ambient

storage temp.

2-8°C

General description

This product is a TCA extract of E. coli serotype O55:B5 (strain CDC 1644-70), which was purified by gel filtration and conjugated to FITC (fluorescein isothiocyanate).

Application

FITC-Lipopolysaccharides (FITC-LPS) may be used to study the movement/transport of LPS across tissue barriers such as the colonic epithelium and cellular binding and internalization of lipopolysaccharides with fluorescence-based assay systems. FITC-LPS may be used to evaluate, qualify and quantify lipopolysaccharides (LPS) removal reagents and procedures. FITC-LPS conjugate may be used for the detection of LPA binding proteins and motifs.
I lipopolisaccaridi (LPS) sono componenti caratteristici della parete cellulare dei batteri gram negativi. Il LPS e la sua frazione lipide A stimolano le cellule del sistema immunitario innato attraverso il recettore Toll-like 4 (TLR4), un elemento della famiglia delle proteine recettoriali Toll-like, che riconosce i profili molecolari associati ai patogeni (PAMP) comuni.

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml), yielding a hazy faint yellow solution. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Questo articolo
L2637L6529L3755
Lipopolisaccaridi FITC conjugate

Sigma-Aldrich

F8666

Lipopolisaccaridi

Lipopolisaccaridi purified by gel-filtration chromatography

Sigma-Aldrich

L2637

Lipopolisaccaridi

Lipopolisaccaridi γ-irradiated, BioXtra, suitable for cell culture

Sigma-Aldrich

L6529

Lipopolisaccaridi

Lipopolysaccharides from Escherichia coli O26:B6 purified by trichloroacetic acid extraction

Sigma-Aldrich

L3755

Lipopolysaccharides from Escherichia coli O26:B6

description

FITC conjugate

description

purified by gel-filtration chromatography

description

γ-irradiated, BioXtra, suitable for cell culture

description

purified by trichloroacetic acid extraction

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O26:B6)

solubility

H2O: 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow

solubility

water: soluble

solubility

H2O: 5 mg/mL, faintly hazy to hazy

solubility

water: soluble

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

color

yellow to orange

color

white to faint gray

color

-

color

white to yellow

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pictograms

Skull and crossbones
GHS06

signalword

Danger

hcodes

H300

Hazard Classifications

Acute Tox. 2 Oral

Classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number

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Domande

  1. What is the molecular weight?

    1 risposta

    1. The molecular weight of this product is not determined. However, LPS is heterogeneous and tends to form aggregates of varying sizes that may range from 1-4 million or greater. When treated with SDS and heat the molecular weight range is approximately 50 to 100 kDa. The FITC used to prepare this product has a molecular weight of 389.38 g/mol. There are approximately 3.6 ug FITC per milligram LPS in this preparation.

      Please see the link below to learn more about Lipopolysaccharides:
      https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

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