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GE17-0300-10

Ficoll® PM 400

Cytiva 17-0300-10, pack of 100 g

Sinonimo/i:

Density gradient medium

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About This Item

Codice UNSPSC:
12352201
NACRES:
NA.25

Stato

solid
spray-dried

Confezionamento

pack of 100 g

Produttore/marchio commerciale

Cytiva 17-0300-10

Colore

White

Solubilità

soluble

Descrizione generale

High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. The molecules are highly branched and the high content of hydroxyl groups leads to very good solubility in aqueous media.
It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained

Caratteristiche e vantaggi

  • For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
  • Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
  • Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
  • Better osmotic properties than sucrose.
  • Preserves functional and morphological integrity.
  • Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
  • Does not penetrate biological membranes.
  • Serves as the raw material for preparation of Ficoll-Paque gradients.
  • Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Ficoll is a registered trademark of Cytiva

Codice della classe di stoccaggio

13 - Non Combustible Solids


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This page shows methods to remove Percoll from a sample after centrifugation.

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Protocolli

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

Contenuto correlato

Data was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.