When trying to isolate mononuclear cells you can use this product but we offer Histopaque that is a mixture of Ficoll and Hypaque. For humans, we offer H8889, Histopaque®-1077 Hybri-Max™ or 1077-1, Histopaque®-1077. For rodents we offer 1083-1, Histopaque®-1083. For isolation of neutrophils, we offer 1119-1, Histopaque®-1119.
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| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
5 g | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 83,80 € |
10 g | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 135,00 € |
25 g | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 251,00 € |
100 g | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 639,00 € |
Informazioni su questo articolo
83,80 €
biological source
non-animal source
Quality Segment
grade
Molecular Biology
product line
BioXtra
form
lyophilized powder
foreign activity
DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected
storage temp.
room temp
General description
Application
- hybridization experiments to reduce non-specific binding of labeled probes to the membrane
- preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
- separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
- producing density gradients to separate subcellular components
- vitrification of cell and tissue samples.
- to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).
Features and Benefits
- free from DNases, RNases, phosphatases and proteases
- preserves functional and morphological integrity of the separated cells
Other Notes
Legal Information
1 of 1
Questo articolo | |||
|---|---|---|---|
| description BioXtra, lyophilized powder | description lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture | description lyophilized powder, Type 400-DL, BioReagent, suitable for cell culture | description BioXtra, Type 400-DL, lyophilized powder |
| grade Molecular Biology | grade - | grade - | grade - |
| biological source non-animal source | biological source - | biological source - | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form - | form lyophilized powder |
| storage temp. room temp | storage temp. - | storage temp. - | storage temp. 15-25°C |
| product line BioXtra | product line BioXtra | product line BioReagent | product line BioXtra |
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Classe di stoccaggio
11 - Combustible Solids
Che succede?
WGK 3
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
DPI (dispositivi di protezione individuale)
Eyeshields, Gloves, type N95 (US)
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Can you use Product F2637, Ficoll, to isolate mononuclear cells?
1 risposta-
Utile?
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Do you have a protocols for use of Product F2637, Ficoll, in various applications?
1 risposta-
Please check the product information sheet for F4375 which has a detailed protocol.
Utile?
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What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile?
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Can Product F2637, Ficoll, be used as a component of a DNA loading buffer?
1 risposta-
This product can be used in a DNA loading buffer with the following recipe to prepare a 6X stock:0.25% bromophenol blue0.25% xylene cyanol FF15% Ficoll
Utile?
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What is the difference between Ficoll 400 and Ficoll PM 400?
1 risposta-
The difference between Ficoll 400 and Ficoll PM 400 is the PM 400 has stricter specifications on the number of microorganisms/g and the endotoxin level is < 0.125 EU/ml
Utile?
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Is Product F2637, Ficoll, the same as Ficoll 400?
1 risposta-
Yes. It is Ficoll 400. This product is graded molecular biology grade and is free of any nucleases
Utile?
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