FD40S

Destrano marcato con isotiocianato di fluoresceina

average mol wt 40,000

• Sinonimo/i: FITC–destrano
• Numero CAS: 60842-46-8
• Numero MDL: MFCD00131092
• Codice UNSPSC: 12352201
• NACRES: NA.25

Proprietà

• Origine biologica: synthetic (organic)
• Coniugato: FITC conjugate
• Stato: powder
• PM: average mol wt 40,000
• Grado di funzionalizzazione: 0.003-0.020 mol FITC per mol glucose
• Colore: yellow to dark orange
• Solubilità: H₂O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% α-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.[1][2] Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million.[3] FITC-dextran has an excitation maximum of 490 nm. It has a pH range of 5 to 9. [4]

Applicazioni

Destrano marcato con isotiocianato di fluoresceina per il possibile utilizzo negli studi di perfusione negli animali.

Fluorescein isothiocyanate–dextran (FITC-dextran, fluorescein-dextran, FITC) was used in the following processes:

• Fluorescein-dextran microscopy.[5][6][7]
• To evaluate the in vivo pulmonary vascular permeability in polymorphonuclear leukocyte α-defensins expressing transgenic mice.[8]
• To quantify endocytic activity by flow cytometry.[9]
• To label α-lactalbumin and ribonuclease.[10]

FITC-dextran is suitable for extensive microcirculation and cell permeability research utilizing microfluorimetry. It is also suitable for the study of plant cell wall porosity and capillary permeability.

Linkage

Similar to FD-40, but produced by Sigma.

Nota sulla preparazione

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Altre note

Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H315,H319,H335
• Consigli di prudenza: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)