The solubility of this enzyme is tested on the finished product at 1 mL per vial in water. The 100-unit package size will be readily soluble in 1 mL, however, the number of actual units per vial should be reviewed in the lot-specific certificate of analysis. The vial content is typically greater than 100 units. The reconstitution volume would have to be adjusted accordingly in order to achieve a 100 unit per milliliter concentration.
See the link below to review the sample Certificate of analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A6237-100UN0000174833.pdf
A6237
Apyrase from potato
recombinant, expressed in Pichia pastoris, ATPase ≥1000 units/mg protein, lyophilized powder
Sinonimo/i:
Apyrase from potato, Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase
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100 UNITS
166,00 €
500 UNITS
609,00 €
166,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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Cambia visualizzazione
100 UNITS
166,00 €
500 UNITS
609,00 €
About This Item
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
166,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Prodotti consigliati
Ricombinante
expressed in Pichia pastoris
Livello qualitativo
Stato
lyophilized powder
ATPase activity
≥1000 units/mg protein
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Descrizione generale
Apyrase, also known as ATP-diphosphohydrolase, is expressed in wide variety of plant and animal tissues. The commercially accessible apyrase enzyme is isolated from potato tubers.[1]
Azioni biochim/fisiol
Apyrase hydrolyses the phosphoanhydride bonds of nucleoside tri- and di-phosphates in the presence of divalent cations.[1][2] It has wide substrate specificity for nucleotides. This property of the enzyme makes it suitable for different biotechnical applications, including DNA sequencing and platelet-aggregation inhibition.[1]
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Certificati d'analisi (COA)
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Hello. Is the concentration of 100 units in 1 mL of water? If not, how can I make the dilution to obtain 100 units? Thanks for you attention! Guilherme
1 risposta-
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