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Merck
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Documenti

A6132

Sigma-Aldrich

Apirasi

ATPase ≥3.0 units/mg protein, lyophilized powder

Sinonimo/i:

Adenosina 5′-difosfatasi, Adenosina 5′-trifosfatasi

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About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

potato

Livello qualitativo

Tipo

Grade I

Forma fisica

lyophilized powder

ATPase activity

≥3.0 units/mg protein

Composizione

Protein, 30-60%

Temperatura di conservazione

−20°C

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Applicazioni

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .
In diverse varietà di S. tuberosum si trovano almeno due isoenzimi: uno con un rapporto ATPasi/ADPasi elevato (∼10) e un altro con un rapporto basso (∼1).
Reazione: ATP → ADP+Pi → AMP+2Pi.

Azioni biochim/fisiol

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Confezionamento

Sold on the basis of ATPase units.

Altre note

Mixture of high and low ratio isoenzymes.

Definizione di unità

One unit will liberate 1.0 micromole of inorganic phosphorus per minute at pH 6.5 at 30 °C

Stato fisico

Partially purified, lyophilized powder containing potassium succinate

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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Qian Wang et al.
Neuroscience bulletin, 37(9), 1303-1313 (2021-06-06)
Food deprivation can rescue obesity and overweight-induced mood disorders, and promote mood performance in normal subjects. Animal studies and clinical research have revealed the antidepressant-like effect of calorie restriction, but little is known about the mechanism of calorie restriction-induced mood
J Vilmart-Seuwen et al.
The Journal of cell biology, 103(4), 1279-1288 (1986-10-01)
We have tried to specify a widespread hypothesis on the requirement of ATP for exocytosis (membrane fusion). With Paramecium tetraurelia cells, synchronously (approximately 1 s) exocytosing trichocysts, ATP pools have been measured in different strains, including wild type cells, "non-discharge"
Avanish Rai et al.
Bio-protocol, 11(1), e3874-e3874 (2021-03-19)
Isoprenoids represent the largest class of metabolites with amazing diversities in structure and function. They are involved in protecting plants against pathogens or herbivores or involved in attracting pollinators. Isoprenoids are derived from geranyl diphosphate (GPP; C10), farnesyl diphosphate (FPP;
I Shirakawa et al.
The Journal of experimental biology, 198(Pt 8), 1809-1815 (1995-08-01)
Kinesin is a motor protein that converts chemical energy derived from ATP hydrolysis into mechanical work to transport cellular components along microtubules. We studied the properties of ATP-dependent microtubule-kinesin sliding with two different in vitro assay systems. In one assay
Robert A Amos et al.
Nature plants, 8(11), 1289-1303 (2022-11-11)
Rhamnogalacturonan I (RG-I) is a major plant cell wall pectic polysaccharide defined by its repeating disaccharide backbone structure of [4)-α-D-GalA-(1,2)-α-L-Rha-(1,]. A family of RG-I:Rhamnosyltransferases (RRT) has previously been identified, but synthesis of the RG-I backbone has not been demonstrated in

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