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Merck
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Documenti

A7646

Sigma-Aldrich

Apirasi

ATPase ≥60 units/mg protein, lyophilized powder (partially purified)

Sinonimo/i:

Adenosina 5′-difosfatasi, Adenosina 5′-trifosfatasi

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About This Item

Numero CAS:
9000-95-7
Classificazione EC (Enzyme Commission):
3.6.1.5 (BRENDA, IUBMB)
Numero CE:
232-569-8
Numero MDL:
MFCD00130542
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

potato

Tipo

Grade III

Forma fisica

lyophilized powder (partially purified)

ATPase activity

≥60 units/mg protein

PM

~49 kDa by gel filtration

Composizione

Protein, 30-60%

Solubilità

H2O: soluble 10 mg/mL, slightly hazy

applicazioni

diagnostic assay manufacturing

Attività estranea

Acid Phosphatase ≤5% of base activity

Temperatura di conservazione

−20°C

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Applicazioni

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.
In diverse varietà di S. tuberosum si trovano almeno due isoenzimi: uno con un rapporto ATPasi/ADPasi elevato (∼10) e un altro con un rapporto basso (∼1).
Reazione: ATP → ADP+Pi → AMP+2Pi.

Azioni biochim/fisiol

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.

Confezionamento

Sold on the basis of ATPase units

Altre note

Mixture of high and low ratio isoenzymes.

Qualità

Contains ≤ 5% acid phosphatase.

Definizione di unità

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Yen-Ta Chen et al.
Journal of tissue engineering and regenerative medicine, 13(12), 2155-2168 (2019-09-11)
This study tested the hypothesis that preactivated and disaggregated shape-changed platelet (PreD-SCP) therapy significantly protected rat kidney from ischemia-reperfusion (IR) injury. Adult-male Sprague-Dawley rats (n = 24) were equally categorized into Groups 1 (sham-operated control [SC]), 2 (SC + PreD-SCP)
H J Kruse et al.
Arteriosclerosis and thrombosis : a journal of vascular biology, 14(11), 1821-1828 (1994-11-01)
The blocker of receptor-mediated calcium entry SK&F 96365 was used to evaluate the contribution of calcium influx to the formation of biologically active endothelial prostanoids and endothelium-derived relaxing factor (EDRF). SK&F 96365 inhibited histamine-stimulated calcium entry into human umbilical vein
Marko Gosak et al.
Life (Basel, Switzerland), 11(5) (2021-05-01)
Ca2+ homeostasis and signaling disturbances are associated with lens pathophysiology and are involved in cataract formation. Here, we explored the spatiotemporal changes in Ca2+ signaling in lens epithelial cells (LECs) upon local mechanical stimulation, to better understand the LECs' intercellular
Xiao-Jing Kuang et al.
Theranostics, 12(8), 3703-3718 (2022-06-07)
Rationale: Stress is a major risk factor for the development of depression. However, the underlying molecular mechanisms of stress vulnerability in depression are largely uncharacterized. Methods: P2X2 receptors (a major receptor for gliotransmitter-ATP) in the medial prefrontal cortex (mPFC) were
Properties of Two Apyrases from Solanum tuberosum
Kettlun, A. et al.
Phytochemistry, 21(3), 551-558 (1982)
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