The Mono-Poly resolving media has only a slight difference in formulation compared to Histopaque 11191, primarily in its density which can vary from 1.112 to 1.116 g/ml. In contrast, the specification for Histopaque 11191 is 1.119 g/ml +/- 0.001 g/ml.
11191
Histopaque®-1119
Histopaque®-1119
sterile-filtered, density: 1.119
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100 ML
159,00 €
6 X 100 ML
732,00 €
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Cambia visualizzazione
100 ML
159,00 €
6 X 100 ML
732,00 €
About This Item
Codice UNSPSC:
12352207
NACRES:
NA.75
Prodotti consigliati
Sterilità
sterile-filtered
Stato
liquid
Durata
Expiry date on the label.
tecniche
cell culture | mammalian: suitable
Impurezze
endotoxin, tested
Densità
1.119
applicazioni
hematology
histology
Temperatura di conservazione
2-8°C
Descrizione generale
L'Histopaque®-1119 è un mezzo sterile, testato per l′assenza di endotossine e pronto all′uso. Si tratta di una soluzione composta da polisaccarosio e sodio diatrizoato portata a una densità di 1,119 g/mL e pH=8,8–9,0.
Applicazioni
Histopaque®-1119 può essere usato insieme a Histopaque-1077 poiché facilita un rapido recupero di cellule mononucleate e granulociti vitali da piccoli volumi di sangue intero o midollo osseo
Caratteristiche e vantaggi
I numerosi vantaggi di Histopaque®-1119 sono:
- permette di recuperare in maniera ottimale le cellule vitali
- consente la separazione selettiva dei diversi tipi di cellule ematiche
- previene la distorsione delle cellule
- preserva la vitalità delle cellule
- l'interferenza da parte di cellule estranee è minima
- offre prestazioni riproducibili tra i lotti
- è filtrato sterilmente e testato per l'assenza di endotossine
- Stabile a 2-8°C per almeno 2 anni se mantenuto al riparo dalla luce
Principio
Il processo comporta la formazione di un doppio gradiente a seguito della stratificazione di un egual volume di Histopaque-1077 al di sopra di Histopaque-1119. Il sangue intero contenente l′anticoagulante viene delicatamente depositato sullo strato superiore di Histopaque-1077. Dopo centrifugazione, gli eritrociti risultano aggregati dal polisaccarosio e perciò sedimentano rapidamente. I granulociti si dispongono a livello dell′interfaccia inferiore formata da Histopaque-1077 e da Histopaque-1119, mentre i linfociti e le altre cellule mononucleate si dispongono a livello dell′interfaccia superiore formata dal plasma e Histopaque-1077. La maggioranza delle piastrine estranee presenti nell′interfaccia superiore può essere rimossa mediante centrifugazione a bassa velocità durante le fasi di lavaggio. La contaminazione eritrocitaria è trascurabile.
Note legali
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
Codice della classe di stoccaggio
8A - Combustible corrosive hazardous materials
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Articoli
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Protocolli
This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
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Is there a product similar to Mono-Poly resolving media?
1 risposta-
Utile?
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Is the Histopaque-1119 solution compatible with blood stored in PAXgene cell-free DNA tubes for separating different cells from the blood when used in combination with Histopaque-1077 for a double gradient?
1 risposta-
The PAX gene Blood DNA tubes were not tested for compatibility with any of the Histopaque products. As a general rule, fresh blood provides the best results with the Histopaque products, and it is generally not recommended to use blood stored for more than 24 hours.
Utile?
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Is it possible to use Histopaque 1119 as a double gradient media with another brand of density gradient media, such as Ficoll paque plus, or does it only work with Histopaque 1077?
1 risposta-
Histopaque and Ficoll Paque are chemically identical solutions. For all practical purposes, both have polysucrose 400.
Utile?
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Are there any comparable products for Pancoll at densities 1.119 g/ml and 1.077 g/ml, to be used in cell isolation for gene expression studies or diagnostics.
1 risposta-
As Pancoll is polysucrose-based, Histopaque and Ficoll Paque have essentially the same formulations. For Histopaque and Ficoll Paque, products are available with densities of 1.073, 1.077, 1.083, 1.084, and 1.119 g/ml. Any of the Histopaque and Ficoll Paque products with densities of 1.077 g/ml and 1.119 g/ml should be suitable crossmatches for Pancoll products with densities of 1.077 g/ml and 1.119 g/ml.
Utile?
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Hi. I am trying to adapt an older protocol that calls for a certain percentage of Ficoll. What percentage does this product contain?
1 risposta-
This product is a mixture of Ficoll (polysucrose 400) and sodium diatrizoate at 60 grams and 167 grams per liter, respectively. The solution may not be a suitable substitute for protocols requiring Ficoll, only. Please see product F5415 to review a 20% Ficoll solution in water. This item may a better option:
https://www.sigmaaldrich.com/product/sial/f5415Other pure Ficoll products are available. See these potential product numbers below:
F4375
F2637
F8016
F9378
F8636
F1418
GE17-0300-50
GE17-0300-10
GE17-0300-05Utile?
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The Product 11191, Histopaque®-1119, froze in transit. Is the product still suitable for use?
1 risposta-
Mix the bottles upon receipt several times to ensure the product is uniform. The products are shipped ambient and gradients in situ can form if the solution freezes during transport. As long as the bottle has not broken and no precipitate is noted, the product is suitable for use after slowly thawing and mixing.
Utile?
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Can the procedure for using Product 11191, Histopaque®-1119, be used successfully with species such as rats and mice?
1 risposta-
The Histopaque 1119-1 procedure was developed for use with human blood. Due to the lower number of circulating neutrophils in rat or mouse peripheral blood, the technique tends not to work as well with rats or mice.
Utile?
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Can the procedure for Product 11191, Histopaque®-1119, be performed using the various Accuspin products?
1 risposta-
No. Accuspin products are intended for use with just a single density gradient - such as Histopaque® 1077.
Utile?
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When using Product 11191, Histopaque®-1119, what is the expected percent recovery for the band containing neutrophils.
1 risposta-
No percent recovery data has been generated. Due to the importance of technique with the Histopaque 1119-1, percent recovery varies with the skill of the operator.
Utile?
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What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile?
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