374805

Hemocyanin, Keyhole Limpet, Megathura crenulata

Hemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

• Sinonimo/i: Hemocyanin, Keyhole Limpet, Megathura crenulata, KLH
• Numero CAS: 9013-72-3
• Numero MDL: MFCD00131280
• Codice UNSPSC: 12352202

Proprietà

• Livello qualitativo: 200
• Descrizione: Merck USA index - 14, 4647
• Saggio: ≥90% (size exclusion chromatography)
• Stato: lyophilized solid
• non contiene: preservative
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Colore: blue to blue-gray
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Hemocyanin purified from Keyhole Limpet (Mega thura crenulata). Protein:≥60%; copper to protein ratio: ≥1.5 x 10^-3

Protein:≥60%; copper to protein ratio: ≥1.5 x 10^-3

Stable solid containing more than 60% protein. Protein is 90% undissociated hemocyanin.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

Lyophilized from BES buffer, MgSO₄.

Ricostituzione

Dissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).

Altre note

Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
Erlanger, B.F. 1980. Methods Enzymol.70, 85.

Protein concentration: ≥60%

Copper: ≥0.09%

Copper to Protein ratio: ≥1.5 x 10^-3

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3