Összes fotó(1)
Fontos dokumentumok
T4330
Turbonuclease from Serratia marcescens
recombinant, expressed in E. coli
Szinonimák:
Endonuclease from Serratia marcescens
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
CAS-szám:
MDL-szám:
UNSPSC kód:
12352204
NACRES:
NA.54
Javasolt termékek
biológiai forrás
Serratia marcescens
Minőségi szint
rekombináns
expressed in E. coli
Forma
liquid
koncentráció
≥200,000 units/mL
technika/technikák
DNA purification: suitable
alkalmasság
suitable for cell lysis
alkalmazás(ok)
life science and biopharma
tárolási hőmérséklet
−20°C
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Általános leírás
Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases.
Alkalmazás
Turbonuclease from Serratia marcescens has been used for cell lysis during proximity biotinylation assay (BioID) and affinity-purification. It has also been used as a component of lysis buffer for protein extraction from cell lines for affinity purification studies.
Turbonuclease has been used in a study to assess the TY3 gag3 spacer effect on intracellular condensation and uncoating.
Used for the removal of nucleic acid from protein samples.
Biokémiai/fiziológiai hatások
Digests native or heat-denatured DNA and RNA.
Endonuclease from Serratia marcescens is effective against both single- and double-stranded DNA and RNA. It mediates the digestion of the 3′ O—P bond resulting in oligonucleotides ending with 5′ monophosphate. The activity of this enzyme is known to be less affected by the reducing and chaotropic agents. It is highly stable at room temperature. This endonuclease eliminates the undesired nucleic acids in downstream processing.
Turbonuclease provides a nuclease treatment by reducing viscosity and degrading RNA, genomic DNA, baculovirus DNA, and unencapsidated vector DNA.
Egység definíció
One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C.
Fizikai forma
Supplied as a solution in 50 mM Tris-HCl, pH 8.0 and 50 mM NaCl
Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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Analitikai tanúsítványok (COA)
Lot/Batch Number
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M D Miller et al.
Journal of molecular biology, 288(5), 975-987 (1999-05-18)
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