SRP5348

GST protein, tag-free

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• CAS-szám: 50812-37-8
• UNSPSC kód: 12352202
• NACRES: NA.32

Tulajdonságok

• rekombináns: expressed in E. coli
• Teszt: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• molekulatömeg: ~25 kDa
• technika/technikák: ligand binding assay: suitable
• NCBI elérési szám: U13850
• kiszállítva: dry ice
• tárolási hőmérséklet: −70°C
• Géninformáció: Escherichia coli IAI39 ... gst> gst; gst(7152360)

Leírás

Általános leírás

Glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial and microsomal proteins that are used to create the so-called GST gene fusion system. These proteins are capable of multiple reactions with a multitude of substrates. They have a role in detoxication and toxification mechanisms. GST tag has the size of 220 amino acids, which is relatively large compared to other tags like the myc- or the FLAG-tag that are quite small. GST can be used to purify and detect proteins of interest. In a GST gene fusion system, an expression vector consists of the gene sequence encoding the protein of interest and GST sequence alongside. After induction, the fusion proteins can be purified using affinity chromatography via its high affinity for glutathione.

Alkalmazás

GST protein, tag-free has been used in in vitro F-actin binding assay. It has also been used as a protein tag for TPX2 (targeting protein for Xklp2).

Fizikai forma

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Elkészítési megjegyzés

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable