SRP4037

FGF-2 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Szinonimák: FGF-2, Fibroblast Growth Factor-basic, HBGF-2, Prostatropin
• MDL-szám: MFCD00081382
• UNSPSC kód: 12352202
• NACRES: NA.32

Tulajdonságok

• biológiai forrás: human
• rekombináns: expressed in E. coli
• Teszt: ≥95% (HPLC); ≥95% (SDS-PAGE)
• form: lyophilized
• molekulatömeg: ~17.2 kDa
• kiszerelés: pkg of 50 μg
• tárolási körülmény: avoid repeated freeze/thaw cycles
• szennyeződések: endotoxin, tested
• NCBI elérési szám: NM_002006
• UniProt elérési szám: P09038
• kiszállítva: wet ice
• tárolási hőmérséklet: −20°C
• Géninformáció: human ... FGF2(2247)

Leírás

Általános leírás

The gene FGF2 (fibroblast growth factor 2) is mapped to human chromosome 4q28. FGF-2 is a basic heparin binding growth factor. The recombinant human FGF-2 is a 17.2kDa protein containing 154 amino acid residues. It is also referred to as BFGF (basic fibroblast growth factor).

Alkalmazás

FGF-2 human has been used:

• as medium supplement for E9.5 neuroepithelial cell culture
• as a component in the neurobasal growth medium
• in growing embryonic neural stem/progenitor cells (NSPCs) in a 2D culture

FGF2 (fibroblast growth factor 2)-human has been used in endothelial colony assay.

Biokémiai/fiziológiai hatások

FGF2 (fibroblast growth factor 2, also referred to as BFGF (basic fibroblast growth factor)) interacts with FGFR1 (fibroblast growth factor receptor 1), FGFR2 and FGFR3. It stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF2 also determines the fate of neural precursor cells and promotes neuronal axon regeneration. It plays an important role in spinal cord injury repair. FGF2 is known to stimulate angiogenesis and is associated with cancer development. High levels of FGF2 are present in the kidney of children with HIV (human immunodeficiency virus)-renal diseases. The FGF2 mRNA is a target of miRNA miR-503.

Fizikai forma

Lyophilized from a 1 mg/ml solution of 5 mM Tris pH 7.6 plus 150 mM NaCl.

Feloldás

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in 5 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable