S8445

SigmaMarker^™

wide range, mol wt 6,500-200,000 Da

• Szinonimák: protein markers, protein standards
• MDL-szám: MFCD00165844
• UNSPSC kód: 41105337
• NACRES: NA.25

Tulajdonságok

• Minőségi szint: 200
• form: lyophilized powder
• használat: sufficient for 10-20 large gels (16 × 18 cm); sufficient for 20-30 mini-gels (10 × 10 cm); vial sufficient for 50-100 PhastGels
• molekulatömeg: 6,500-200,000 Da
• tárolási hőmérséklet: 2-8°C

Leírás

Alkalmazás

SigmaMarker has been used as a protein marker in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE).

SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining.

Egyéb megjegyzések

Protein (mol. wt.)
Aprotinin, bovine lung (6,500)
α-Lactalbumin, bovine milk (14,200)
Trypsin inhibitor, soybean (20,000)
Trypsinogen, bovine pancreas (24,000)
Carbonic anhydrase, bovine erythrocytes (29,000)
Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)
Ovalbumin, chicken egg (45,000)
Glutamic dehydrogenase, bovine liver (55,000)
Albumin, bovine serum (66,000)
Phosphorylase B, rabbit muscle (97,000)
β-Galactosidase, E. coli (116,000)
Myosin, porcine heart (200,000)

Will yield 12 protein bands with Coomassie Blue staining.

Feloldás

Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 10 mM TCEP, 2% SDS, and 0.01% bromophenol blue.

Jogi információk

SigmaMarker is a trademark of Sigma-Aldrich Co. LLC

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H315,H317,H319,H334
• Óvintézkedésre vonatkozó mondatok: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Veszélyességi osztályok: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: dust mask type N95 (US), Eyeshields, Faceshields, Gloves