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PKH67GL

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Szinonimák:

Green PKH membrane labeling kit

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Összes fotó(2)

About This Item

UNSPSC kód:
12352207
NACRES:
NA.32

Minőségi szint

200

kiszerelés

pkg of 1 kit

tárolási körülmény

protect from light

fluoreszcencia

λex 490 nm; λem 502 nm (PKH67 dye)

észlelési módszer

fluorometric

kiszállítva

ambient

tárolási hőmérséklet

room temp

Alkalmazás

PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling has been used:
  • To label and further investigate the exosomes expelled from cells in triple-negative breast cancer condition.
  • To label apoptotic cells, in order to study the role of ανβ5 receptor in both binding and internalization of apoptotic cells.
  • In fluorescence imaging.

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Kapcsolódás

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Jogi információk

CellVue is a registered trademark of Phanos Technologies

Kit Components Only

Product No.
Leírás
  • Diluent C 6 x 10
  • PKH67 Cell Linker in ethanol .5 mL

Piktogramok

FlameExclamation mark
GHS02,GHS07

Figyelmeztetés

Danger

Figyelmeztető mondatok

H225,H319

Óvintézkedésre vonatkozó mondatok

P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338

Veszélyességi osztályok

Eye Irrit. 2 - Flam. Liq. 2

Tárolási osztály kódja

3 - Flammable liquids

Lobbanási pont (F)

57.2 °F - closed cup

Lobbanási pont (C)

14 °C - closed cup

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
MKCV4102
MKCQ9079
MKCP3216
MKCQ0194
MKCK9600

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COA keresése

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Anjali P Kusumbe et al.
Stem cells (Dayton, Ohio), 27(3), 498-508 (2009-03-04)
Recruitment and localization of endothelial precursors within tumors is a potential area for the development of therapeutics, because their functional contribution to tumor vasculature is realized to be important for cancer cell survival. However, the exact nature of the recruited
Exosomes from triple-negative breast cancer cells can transfer phenotypic traits representing their cells of origin to secondary cells
O Brien K, et al.
European Journal of Cancer, 49(8), 1845-1859 (2013)
Increased expression of inducible HSP70 in apoptotic cells is correlated with their efficacy for antitumor vaccine therapy
Masse D, et al.
International Journal of Cancer. Journal International Du Cancer, 111(4), 575-583 (2004)
alpha v beta 5 integrin recruits the CrkII-Dock180-Rac1 complex for phagocytosis of apoptotic cells
Albert, Matthew L and Kim, Jong-Ii and Birge, Raymond B
Nature Cell Biology, 2(12), 899-899 (2000)
Nobuyoshi Kosaka et al.
The Journal of biological chemistry, 287(2), 1397-1405 (2011-11-30)
Normal epithelial cells regulate the secretion of autocrine and paracrine factors that prevent aberrant growth of neighboring cells, leading to healthy development and normal metabolism. One reason for tumor initiation is considered to be a failure of this homeostatic cell
View All Related Papers

Cikkek

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Összes mutatása

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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