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MINI67

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Szinonimák:

Green PKH membrane mini labeling kit

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
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About This Item

UNSPSC kód:
12352207
NACRES:
NA.32

kiszerelés

pkg of 1 kit

Minőségi szint

200

gyártó/kereskedő neve

Distributed for Phanos Technologies

tárolási körülmény

protect from light

technika/technikák

flow cytometry: suitable

fluoreszcencia

λex 490 nm; λem 502 nm (PKH67 dye)

alkalmazás(ok)

cell analysis
detection

észlelési módszer

fluorometric

kiszállítva

ambient

tárolási hőmérséklet

room temp

Általános leírás

PKH67 kit is for general cell membrane labeling. It has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Alkalmazás

PKH67 green fluorescent cell linker mini kit for general cell membrane labeling has been used to stain:
  • exosomes
  • pericytes
  • UT-7/TPO cells

This kit is for general cell membrane labeling. Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes.

Kapcsolódás

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Jogi információk

Distributed for Phanos Technologies, Inc.
CellVue is a registered trademark of Phanos Technologies

Kit Components Only

Product No.
Leírás
  • Diluent C 10 mL
  • PKH67 Cell Linker in ethanol 0.1 mL

Piktogramok

FlameExclamation mark
GHS02,GHS07

Figyelmeztetés

Danger

Figyelmeztető mondatok

H225,H319

Óvintézkedésre vonatkozó mondatok

P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338

Veszélyességi osztályok

Eye Irrit. 2 - Flam. Liq. 2

Tárolási osztály kódja

3 - Flammable liquids

Lobbanási pont (F)

55.4 °F - closed cup

Lobbanási pont (C)

13 °C - closed cup

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
MKCV7532
MKCT3677
MKCP8297
MKCK0731
MKCP5319

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COA keresése

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Exosomal MALAT1 derived from oxidized low-density lipoprotein-treated endothelial cells promotes M2 macrophage polarization
Huang C, et al.
Molecular Medicine Reports, 18(1), 509-515 (2018)
A microfluidic study of megakaryocytes membrane transport properties to water and dimethyl sulfoxide at suprazero and subzero temperatures
Tseng HY, et al.
Biopreservation and Biobanking, 9(4), 355-362 (2011)
Yuan Yang et al.
Experimental and therapeutic medicine, 20(6), 257-257 (2020-11-18)
Mesenchymal stem cell (MSC) infusion has become a novel therapeutic strategy for complex autoimmune diseases; however, few detailed studies have been performed to investigate the benefit and mechanism of MSC treatment on systemic sclerosis (SSc). The present study aimed to
Simone Moertl et al.
International journal of molecular sciences, 21(7) (2020-04-02)
Normal tissue toxicity is a dose-limiting factor in radiation therapy. Therefore, a detailed understanding of the normal tissue response to radiation is necessary to predict the risk of normal tissue toxicity and to development strategies for tissue protection. One component
Haiyang Xu et al.
Stem cell research & therapy, 10(1), 381-381 (2019-12-18)
Mesenchymal stem cells (MSCs) play a significant role in cancer initiation and metastasis, sometimes by releasing exosomes that mediate cell communication by delivering microRNAs (miRNAs). This study aimed to investigate the effects of exosomal miR-133b derived from MSCs on glioma
View All Related Papers

Cikkek

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Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

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Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

PKH and CellVue® Claret Fluorescent Cell Linker Dyes

PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.

Összes mutatása

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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