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Merck
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Fontos dokumentumok

N3910

Sigma-Aldrich

Neutralization Solution B

Szinonimák:

DNA neutralization solution, neutralization buffer DNA extraction

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12161703
NACRES:
NA.25

grade

for molecular biology

Minőségi szint

Forma

liquid

technika/technikák

DNA amplification: suitable
DNA extraction: suitable

tárolási hőmérséklet

−20°C

Általános leírás

This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed.

Alkalmazás

Neutralization Solution B has been used in the modified Sigma Extract-N-Amp extraction procedure. It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp PCR Reaction Mix.

Jogi információk

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Piktogramok

Exclamation markEnvironment

Figyelmeztetés

Warning

Figyelmeztető mondatok

Veszélyességi osztályok

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


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Analitikai tanúsítványok (COA)

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Dokumentumtár megtekintése

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A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
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Pharmacology, biochemistry, and behavior, 86(2), 327-333 (2006-12-16)
Adolescence is an important stage of brain development. Recent studies have indicated that neurogenesis in the brain occurs throughout life prompting comparisons of adolescent and adult neurogenesis. Since insulin-like growth factor 1 (IGF-1) has been implicated in promoting neurogenesis we
Yan Zhang et al.
American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed

Protocols

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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