Összes fotó(1)
Fontos dokumentumok
MSST0002
SILu™Lite APOA1 Apolipoprotein A-1 human
recombinant, expressed in HEK 293 cells, MS Protein Standard
Szinonimák:
SILuLite Apolipoprotein A-1
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
23201100
NACRES:
NA.12
Javasolt termékek
biológiai forrás
human
Minőségi szint
rekombináns
expressed in HEK 293 cells
tag
His tagged
V5 tagged
Teszt
≥98% (SDS-PAGE)
Forma
lyophilized powder
kiszerelés
vial of 50-65 μg (Lot-specific vial content given on certificate of analysis)
technika/technikák
mass spectrometry (MS): suitable
UniProt elérési szám
tárolási hőmérséklet
−20°C
Géninformáció
human ... APOA1(335)
Related Categories
Biokémiai/fiziológiai hatások
Apolipoprotein A-1(Apo-A1), is a major protein component of high density lipoprotein (HDL)in plasma1. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters2. Apo-A1 was shown to assist in the improvement of predicting cardiovascular events3. The gene that encodes to Apolipoprotein A-1 is APOA1. This gene is closely linked with two other apolipoprotein genes on chromosome 114. Defects in this gene are associated with HDL deficiencies, including Tangier disease5, and with systemic non-neuropathic amyloidosis6. The protein is made up of one major isoform (pI 5.6) and two minor isoforms (pI 5.53 and 5.46). Apo-A1 shows a high content of a-helix structure6. The amphipathic regions in the α-helix structure seem to be responsible for lipid binding capacity. In aqueous solution, Apo-A1 shows self-association with minor conformational change7. In addition, it has been shown that Apo-A1 is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin8.
Fizikai forma
Supplied as a lyophilized powder containing phosphate buffered saline.
Analízis megjegyzés
General Description
SILuLite APOA1 is a recombinant human protein expressed in human 293 cells. It is a monomer of 286 amino acids (including N-terminal signal sequence and C-terminal polyhistidine and V5 tags), with a molecular weight of 32.1 kDa. SILuLite APOA1 is an analytical standard designed to be used as starting material for preparation of calibrators and controls in LC-MS applications.
Sequence
RHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQSDPSRGPFEGKPIPNPLLGLDSTRTGHHHHHHHHGGQ
The N-terminal signal peptide and C-terminal linker peptide, V5 and polyhistidine tags are italicized.
Other Characterization
Suggested Quantitative Analysis Parameters
(MRM settings provided for three suggested peptides)
SILuLite APOA1 is a recombinant human protein expressed in human 293 cells. It is a monomer of 286 amino acids (including N-terminal signal sequence and C-terminal polyhistidine and V5 tags), with a molecular weight of 32.1 kDa. SILuLite APOA1 is an analytical standard designed to be used as starting material for preparation of calibrators and controls in LC-MS applications.
Sequence
RHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQSDPSRGPFEGKPIPNPLLGLDSTRTGHHHHHHHHGGQ
The N-terminal signal peptide and C-terminal linker peptide, V5 and polyhistidine tags are italicized.
Other Characterization
- Sequence confirmed by intact mass analysis
- Identity verified by peptide mapping
- purity >98% (SDS-PAGE)
- Vial content was determined by the Bradford method using BSA as a calibrator. The correction factor of Bradford-to-AAA is 88.33%
Suggested Quantitative Analysis Parameters
(MRM settings provided for three suggested peptides)
Jogi információk
SILu is a trademark of Sigma-Aldrich Co. LLC
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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Analitikai tanúsítványok (COA)
Lot/Batch Number
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B Lamarche et al.
Clinica chimica acta; international journal of clinical chemistry, 286(1-2), 145-161 (1999-10-08)
Reduced plasma high-density lipoprotein (HDL) cholesterol levels have been recognized as a highly significant independent risk factor for atherosclerotic cardiovascular disease. HDL levels are also inversely related to plasma triglyceride levels and there is a dynamic interaction between HDL and
E M Rubin et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(2), 434-438 (1991-01-15)
In Western societies high density lipoprotein (HDL) levels correlate inversely with the risk for coronary heart disease. The primary protein component of both human and mouse HDL is apolipoprotein A-I (apoAI), which comprises greater than 70% of HDL protein and
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