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MDQ1

Sigma-Aldrich

Imprint® Methylated DNA Quantification Kit

To measure global DNA methylation shifts from as low as 10 ng DNA

Szinonimák:

DNA Methylation Quantification, DNA methylation shift

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)

About This Item

UNSPSC kód:
41116158
NACRES:
NA.54

Általános leírás

Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.

Alkalmazás

Imprint® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.

Tulajdonságok és előnyök

  • ELISA based format
  • Procedure completes in 4 easy to follow steps
  • All reagents supplied including control methylated DNA
  • Detection limit is 5 ng of fully methylated DNA
  • Input DNA may be as low as 10-200 ng
  • Procedure completes in 3.5 hours
  • 96 well format allows option of studying single samples or high-throughput studies
  • Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
  • May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum

Jogi információk

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Leírás
Biztonsági adatlap
  • M8570Methylated Control DNA, 50 ng/μLBiztonsági adatlap

kapcsolódó termék

Product No.
Leírás
Árazás

H9161

Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

H9286

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit, 2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

H7162

Anti-methyl-Histone H3 (Me-Lys9) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

H0913

Anti-acetyl-Histone H3 (Ac-Lys9) antibody, Mouse monoclonal, clone AH3-120, purified from hybridoma cell culture

H2663

Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal, clone HDAC2-62, purified from hybridoma cell culture

R4279

Anti-RbAp46, C-terminal antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

MOD50

Imprint® DNA Modification Kit, For bisulfite DNA conversion & purification

H7789

Anti-acetyl-Histone H1.4 (Ac-Lys26) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

CHP1

Imprint® Chromatin Immunoprecipitation Kit, Complete ChIP reaction in 6 hours in flexible strip well format

SH3011

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Epigenetic Regulators

M8570

Methylated Control DNA, 50 ng/μL

Piktogramok

CorrosionExclamation mark
GHS05,GHS07

Figyelmeztetés

Warning

Figyelmeztető mondatok

H290,H317,H412

Óvintézkedésre vonatkozó mondatok

P234 - P261 - P272 - P273 - P280 - P302 + P352

Veszélyességi osztályok

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Tárolási osztály kódja

8A - Combustible corrosive hazardous materials

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Felicitas Thol et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 29(21), 2889-2896 (2011-06-15)
To study the incidence and prognostic impact of mutations in DNA methyltransferase 3A (DNMT3A) in patients with acute myeloid leukemia. A total of 489 patients with AML were examined for mutations in DNMT3A by direct sequencing. The prognostic impact of
Roman Thaler et al.
The British journal of nutrition, 101(5), 743-749 (2008-08-08)
The impact of nutrition on the epigenetic machinery has increasingly attracted interest. The aim of the present study was to demonstrate the effects of various diets on methylation and gene expression. The antioxidative enzyme mitochondrial superoxide dismutase (MnSOD) was chosen
Alok Mishra et al.
Methods in molecular biology (Clifton, N.J.), 863, 15-31 (2012-02-24)
Epigenetics is an emerging science that can help to explain carcinogenesis. The possibility that carcinogenesis may originate in a stem cell process was proposed recently. Stem cells are generated and contribute to tumor formation during the process of tumor development.
Giulia F Del Gobbo et al.
Clinical epigenetics, 10, 34-34 (2018-03-23)
5,10-Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in one-carbon metabolism that ensures the availability of methyl groups for methylation reactions. Two single-nucleotide polymorphisms (SNPs) in the In 303 placentas screened for this study, we observed no significant association between the
Rauf Ahmad Najar et al.
The Journal of nutritional biochemistry, 53, 121-132 (2017-12-09)
The present study has been designed to determine the effect of folate modulation (deficiency/supplementation) with aging on the promoter methylation of tumor suppressor and proto-oncogenes to understand the underlying mechanism of epigenetic alterations. Folate deficiency was induced for 3 and
View All Related Papers

Cikkek

Global DNA Methylation Quantification

Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.

Globális DNS-metiláció kvantitatív meghatározása

A Global DNA Modification Kit (MDQ1) a DNS-metilációt szendvics ELISA-szerű teszttel ellenőrzi, segítve az epigenetikai kutatásokat.

Protocols

Imprint® Methylated DNA Quantification Kit (MDQ1) Protocol

Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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