H7162

Anti-methyl-Histone H3 (Me-Lys⁹) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Szinonimák: Anti-H3K9me1
• MDL-szám: MFCD04974759
• UNSPSC kód: 12352203
• NACRES: NA.41
• konjugátum: unconjugated
• application: ARR; WB
• klón: polyclonal
• faj reaktivitás: Drosophila, Caenorhabditis elegans, rat, bovine, human, mouse, frog, chicken
• citations: 4
• technika/technikák: microarray: suitable; western blot: 1:1,000 using whole cell extract of the human epitheloid carcinoma HeLa cell line

Tulajdonságok

• biológiai forrás: rabbit
• konjugátum: unconjugated
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• Forma: buffered aqueous solution
• faj reaktivitás: Drosophila, Caenorhabditis elegans, rat, bovine, human, mouse, frog, chicken
• technika/technikák: microarray: suitable; western blot: 1:1,000 using whole cell extract of the human epitheloid carcinoma HeLa cell line
• UniProt elérési szám: P68431
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: monomethylation (Lys9)
• Géninformáció: human ... H3F3A(3020) , H3F3B(3021) , HIST1H3A(8350) , HIST1H3B(8358) , HIST1H3C(8352) , HIST1H3D(8351) , HIST1H3E(8353) , HIST1H3F(8968) , HIST1H3G(8355) , HIST1H3H(8357) , HIST1H3I(8354) , HIST1H3J(8356) , HIST2H3A(333932) , HIST2H3C(126961) , HIST3H3(8290) ; mouse ... H3f3a(15078) , H3f3b(15081) , Hist1h3a(360198) , Hist1h3b(319150) , Hist1h3c(319148) , Hist1h3d(319149) , Hist1h3e(319151) , Hist1h3f(260423) , Hist1h3g(97908) , Hist1h3h(319152) , Hist1h3i(319153) , Hist2h3b(319154) , Hist2h3c1(15077) , Hist2h3c2(97114) ; rat ... H3f3b(117056)

Leírás

Általános leírás

Histones are proteins that form the nucleosome along with DNA. A nucleosome consists of core histone octamers made of two heterodimers of H2A and H2B along with two heterodimers of H3 and H4. This octamer is bound by the DNA and H1 protein. The HIST3H3 gene is mapped on the human chromosome at 1q42.13.

Egyediség

Anti-Methyl-Histone H3 [Me-Lys9] recognizes histone H3 methylated on Lys9.

Immunogén

synthetic methylated peptide corresponding to amino acids 7-20 [Me-Lys⁹] of human histone H3. The sequence is identical in many species.

Alkalmazás

Anti-methyl-Histone H3 (Me-Lys9) antibody produced in rabbit may be used in immunoblotting.

Biokémiai/fiziológiai hatások

Histones H3 and H4 are the predominant histones modified by methylation and are highly methylated in mammalian cells. Lysine residues can be mono-, di-, and tri-methylated, adding further complexity to the regulation of chromatin structure. Conserved lysine residues in the N-terminal tail domains of histone H3, Lys-4, Lys-9 and Lys-27 are the preferred sites of methylation. Methylation of H3 at Lys-9 is a modification intrinsically linked to epigenetic silencing and heterochromatin assembly. Histone H3 is methylated at Lys-9 by site-specific H3 methyltransferases (HMTases) and generates a binding site for heterochromatin protein 1 (HP1).

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Tárolás és stabilitás

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.