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Merck

MBD0013

Sigma-Aldrich

Microbial DNA standard from Escherichia coli

Suitable for PCR, sequencing and NGS, 10 ng/μL

Szinonimák:

E.Coli

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
41105500
NACRES:
NA.51

Minőségi szint

form

liquid

koncentráció

10 ng/μL

technika/technikák

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

alkalmasság

suitable for restriction endonuclease digests, PCR amplification, Southern blots, and sequencing reactions

kiszállítva

ambient

tárolási hőmérséklet

−20°C

Általános leírás

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Escherichia coli genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs. Escherichia coli is a gram negative, facultative aerobic, rod-shaped coliform bacterium. E. coli colonizes the infant gut within hours of birth and establishes itself as the most abundant facultative anaerobe of the human intestinal microflora for the remainder of life, equipped with the abilities to grow in the ever-changing environment in the gut and cope with the mammalian host interaction. Nevertheless, E. coli can survive in many different ecological habitats, including abiotic environments, and is considered a highly versatile species. Known habitats of E. coli include soil, water, sediment, and food. Some strains of E. coli have evolved and adapted to a pathogenic lifestyle and can cause different disease pathologies.


Read here how to use our standards to ensure data integrity for your microbiome research.

Alkalmazás

The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0. It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS

Tulajdonságok és előnyök

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Fizikai forma

Liquid - The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0

Egyéb megjegyzések

Strain: MG1655

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

James B Kaper et al.
Nature reviews. Microbiology, 2(2), 123-140 (2004-03-26)
Few microorganisms are as versatile as Escherichia coli. An important member of the normal intestinal microflora of humans and other mammals, E. coli has also been widely exploited as a cloning host in recombinant DNA technology. But E. coli is
Lisa C Crossman et al.
Journal of bacteriology, 192(21), 5822-5831 (2010-08-31)
In most cases, Escherichia coli exists as a harmless commensal organism, but it may on occasion cause intestinal and/or extraintestinal disease. Enterotoxigenic E. coli (ETEC) is the predominant cause of E. coli-mediated diarrhea in the developing world and is responsible
Order and disorder during Escherichia coli divergence.
Heather Hendrickson
PLoS genetics, 5(1), e1000335-e1000335 (2009-01-24)
J Paul Brooks et al.
BMC microbiology, 15, 66-66 (2015-04-17)
Characterizing microbial communities via next-generation sequencing is subject to a number of pitfalls involving sample processing. The observed community composition can be a severe distortion of the quantities of bacteria actually present in the microbiome, hampering analysis and threatening the

Cikkek

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

A DNS-szabványok fokozzák a metagenomikai kutatások integritását, pontos fajvizsgálatot és vegyes közösségi szabványokat kínálva.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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